■肠道菌群影响食物过敏。我们表明,天然化合物小檗碱减少IgE和其他人报道,BBR改变肠道微生物群暗示在BBR功能的微生物群变化的潜在作用。
■我们试图使用小鼠模型评估口服含小檗碱的天然药物与煮花生口服免疫疗法(BNP)方案作为食物过敏的治疗方法,并探索治疗引起的相关性肠道微生物群变化与治疗结果。
■花生过敏(PA)小鼠,用烤花生和霍乱毒素口服致敏,接受口服BNP或对照治疗。PA小鼠接受定期的治疗后烤花生暴露。通过症状可视化和体温测量来评估过敏反应。通过ELISA测量组胺和血清花生特异性IgE水平。通过流式细胞术评估脾IgE+B细胞。通过IlluminaMiSeq将粪便沉淀用于对细菌16SrDNA进行测序。使用内置分析平台分析测序数据。
■BNP治疗方案诱导对花生的长期耐受,伴随着IgE的深度和持续降低,症状评分,血浆组胺,体温,和IgE+B细胞的数量(p<0.001vs.假的)。在各治疗组之间观察到厚壁菌/拟杆菌比率的显著差异。与攻击后组胺和PN-IgE呈正相关的细菌属包括Lachnospileaceae,Ruminocycaceae,和氢氧细菌(所有Firmicutes),而疣菌。皂化产物,肠杆菌科,与拟杆菌呈负相关。
BNP是一种有前途的食物过敏治疗方案,其在小鼠模型中的益处与独特的微生物群特征相关。
Gut microbiota influence food allergy. We showed that the natural compound berberine reduces IgE and others reported that BBR alters gut microbiota implying a potential role for microbiota changes in BBR function.
We sought to evaluate an oral Berberine-containing natural medicine with a boiled peanut oral immunotherapy (BNP) regimen as a treatment for food allergy using a murine model and to explore the correlation of treatment-induced changes in gut microbiota with therapeutic outcomes.
Peanut-allergic (PA) mice, orally sensitized with roasted peanut and cholera toxin, received oral BNP or control treatments. PA mice received periodic post-therapy roasted peanut exposures. Anaphylaxis was assessed by visualization of symptoms and measurement of body temperature. Histamine and serum peanut-specific IgE levels were measured by ELISA. Splenic IgE+B cells were assessed by flow cytometry. Fecal pellets were used for sequencing of bacterial 16S rDNA by Illumina MiSeq. Sequencing data were analyzed using built-in analysis platforms.
BNP treatment regimen induced long-term tolerance to peanut accompanied by profound and sustained reduction of IgE, symptom scores, plasma histamine, body temperature, and number of IgE+ B cells (p <0.001 vs Sham for all). Significant differences were observed for Firmicutes/Bacteroidetes ratio across treatment groups. Bacterial genera positively correlated with post-challenge histamine and PN-IgE included Lachnospiraceae, Ruminococcaceae, and Hydrogenanaerobacterium (all Firmicutes) while Verrucromicrobiacea. Caproiciproducens, Enterobacteriaceae, and Bacteroidales were negatively correlated.
BNP is a promising regimen for food allergy treatment and its benefits in a murine model are associated with a distinct microbiota signature.