Abstract：
Peanut allergy has garnered worldwide attention due to its high incidence rate and severe symptoms, stimulating the demand for the ultrasensitive detection method of peanut allergen. Herein, we successfully developed a novel electrochemical aptasensor for ultrasensitive detection Ara h1, a major allergenic protein present in peanuts. A conductive nickel atoms Anchored Hydrogen-Bonded Organic Frameworks (PFC-73-Ni) were utilized as excellent electrocatalysts toward hydroquinone (HQ) oxidation to generate a readable current signal. The developed electrochemical aptasensor offers wide linear range (1-120 nM) and low detection limit (0.26 nM) for Ara h1. This method demonstrated a recovery rate ranging from 95.00% to 107.42% in standard addition detection of non-peanut food samples. Additionally, the developed electrochemical method was validated with actual samples and demonstrated good consistency with the results obtained from a commercial ELISA kit. This indicates that the established Ara h1 detection method is a promising tool for peanut allergy prevention.
摘要：
花生过敏因其发病率高、症状严重而受到全世界的关注,激发了对花生过敏原超灵敏检测方法的需求。在这里，我们成功开发了一种新型的电化学传感器，用于超灵敏检测花生中存在的主要致敏蛋白Arah1。导电镍原子锚定的氢键有机框架（PFC-73-Ni）被用作对氢醌（HQ）氧化的优异电催化剂，以产生可读的电流信号。开发的电化学传感器为Arah1提供宽线性范围（1-120nM）和低检测限（0.26nM）。在非花生食品样品的标准添加检测中，该方法的回收率为95.00％至107.42％。此外,所开发的电化学方法已在实际样品中进行了验证，并与从商业ELISA试剂盒获得的结果具有良好的一致性。这表明所建立的Arah1检测方法是一种有前途的花生过敏预防工具。
