关于减肥手术(BS)后肠道微生物群在体重减轻中的作用的证据越来越多。本研究的目的是观察袖状胃切除术(SG)和SG加干迷走神经切断术(SG-TV)后肠道菌群的变化,并确定可能有助于改善手术后肥胖的特定微生物。
将40只高脂饮食诱导的肥胖(DIO)小鼠随机分为SG,SG-TV,或假手术(SH)组。在手术前和术后1、2、4、8和12周测量体重(BW)和空腹血糖(FBG)。在手术前和术后第12周收集粪便样品,并使用16SrRNA相对和绝对定量测序进行分析。
手术后,与SH相比,SG和SG-TV手术显着降低了BW和FBG水平,SG-TV取得了比SG更好的效果。手术后观察到肠道菌群的α多样性呈下降趋势,分类组成发生了显着变化。然后,我们确定了一组微生物和途径在BS后的丰度显著不同。SG-TV组的副杆菌属和一个途径(聚酮糖单元生物合成)特别增加,与BW和FBG呈负相关。
SG和SG-TV确实达到了减肥的效果,但是电视可以增强SG的功效。确定的不同微生物和途径,比如副杆菌属,聚酮化合物糖单位生物合成,可能部分介导BS的有益影响,因此可能有助于开发新的基于细菌的治疗方法。
Evidences about the gut microbiota role in weight loss after bariatric surgery (BS) are growing. The objective of this study was to observe the changes of gut microbiota after sleeve gastrectomy (SG) and SG plus truncal vagotomy (SG-TV) and identify specific microbes that may contribute to the improvement of obesity after surgeries.
Forty high-fat diet-induced obesity (DIO) mice were randomized to SG, SG-TV, or sham operation (SH) groups. Body weight (BW) and fast blood glucose (FBG) were measured before and 1, 2, 4, 8, and 12 weeks post-operatively. Fecal samples were collected before and at post-operative week 12 and profiled using 16S rRNA relative and absolute quantitative sequencing.
After the surgery, the SG and SG-TV surgeries significantly reduce BW and FBG levels compared with SH, and the SG-TV achieved better effects than SG. A decreasing trend in alpha diversity of gut microbiota and significant changes in taxonomic composition were observed after surgeries. Then, we identified a set of microbes and pathways significantly different in abundance after BS. The genus
Parabacteroides and one pathway (polyketide sugar unit biosynthesis) increased in SG-TV group specially, which was also negatively correlated with BW and FBG.
SG and SG-TV indeed achieve effects of weight loss, but TV could enhance the efficacy of SG. The identified different microbes and pathways, like
Parabacteroides, polyketide sugar unit biosynthesis, may partly mediate the beneficial effects of BS, and thus possibly contribute to the development of novel bacteria-based therapeutic approaches.