两株命名为c1T和c7T,从惠州市生活垃圾处理厂的垃圾渗滤液中分离出来,广东省,公关中国。两种菌株的细胞都是有氧的,杆状,在Reasoner的2A琼脂平板上不运动并形成黄色菌落。菌株c1T在10-42°C生长(最佳,30°C),pH4.5-10.5(最佳,pH7.0)和0-2.0%(w/v)NaCl(最佳,0-0.5%)。菌株c7T在10-42°C生长(最佳,30°C),pH4.5-10.5(最佳,pH6.0)和0-2.0%(w/v)NaCl(最佳,0-0.5%)。系统发育分析表明,菌株c1T和c7T属于Novosphingoum属。菌株c1T和c7T的16SrRNA基因序列相似性与Novosphingoum种的类型菌株分别为94.5-98.2%和94.3-99.1%,分别。计算的菌株c1T之间的成对平均核苷酸同一性值,c7T和参考菌株在75.2-85.9%的范围内,计算的菌株c1T之间的成对平均氨基酸同一性值,c7T和参考菌株在72.0-88.3%范围内。它们的主要呼吸醌是Q-10,主要的细胞脂肪酸是C18:1ω7c,C18:0,C16:1ω7c,C16:0和C14:02OH。菌株c1T和c7T的主要极性脂质是磷脂酰乙醇胺,二磷脂酰甘油,磷脂酰甘油,鞘糖脂,未知的脂质和未知的磷脂。基于表型,化学分类学,这项研究的系统发育和基因组结果,菌株c1T和c7T应该代表两个独立的新种,其名称为Novosphingobiumpercolationissp。11月。(类型菌株c1T=GDMCC1.2555T=KCTC82826T)和回珠夜蛾。11月。(提出了类型应变c7T=GDMCC1.2556T=KCTC82827T)。菌株c1T和c7T的基因功能注释结果表明,它们可能在降解有机污染物中发挥重要作用。
Two strains designated as c1T and c7T, were isolated from the landfill leachate of a domestic waste treatment plant in Huizhou City, Guangdong Province, PR
China. The cells of both strains were aerobic, rod-shaped, non-motile and formed yellow colonies on Reasoner\'s 2A agar plates. Strain c1T grew at 10-42 °C (optimum, 30 °C), pH 4.5-10.5 (optimum, pH 7.0) and 0-2.0 % (w/v) NaCl (optimum, 0-0.5 %). Strain c7T grew at 10-42 °C (optimum, 30 °C), pH 4.5-10.5 (optimum, pH 6.0) and 0-2.0 % (w/v) NaCl (optimum, 0-0.5 %). Phylogenetic analyses revealed that strains c1T and c7T belong to the genus
Novosphingobium. The 16S rRNA gene sequence similarities of strains c1T and c7T to the type strains of
Novosphingobium species were 94.5-98.2 % and 94.3-99.1 %, respectively. The calculated pairwise average nucleotide identity values among strains c1T, c7T and the reference strains were in the range of 75.2-85.9 % and the calculated pairwise average amino acid identity values among strains c1T, c7T and reference strains were in the range of 72.0-88.3 %. Their major respiratory quinone was Q-10, and the major cellular fatty acids were C18 : 1 ω7c, C18 : 0, C16 : 1 ω7c, C16 : 0 and C14 : 0 2OH. The major polar lipids of strains c1T and c7T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, sphingoglycolipid, unidentified lipids and unidentified phospholipid. Based on phenotypic, chemotaxonomic, phylogenetic and genomic results from this study, strains c1T and c7T should represent two independent novel species of Novosphingobium, for which the names
Novosphingobium percolationis sp. nov. (type strain c1T=GDMCC 1.2555T=KCTC 82826T) and
Novosphingobium huizhouense sp. nov. (type strain c7T=GDMCC 1.2556T=KCTC 82827T) are proposed. The gene function annotation results of strains c1T and c7T suggest that they could play an important role in the degradation of organic pollutants.