Abstract：
A novel Novosphingobium species, designated strain B2638T, was isolated from mangrove sediments which was collected from Beibu Gulf, Beihai, P. R. China. The isolate could grow in the presence of chlorpyrifos. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the isolate belonged to the genus Novosphingobium, showing 99.9% sequence similarity with N. decloroationis 502str22T and less than 98% similarity with other type strain of species of this genus. Molecular typing by BOX-PCR divided strain B2638T and N. declorationis 502str22T into two clusters and indicated that they were not identical. Genomic comparison referenced by values of the DNA-DNA hybridization (dDDH) and the average nucleotide identity (ANI) between strain B2638T and its close phylogenetic neighbors were 20.0-29.5% and 75.3-85.3%, respectively, that were lower than proposed thresholds for bacterial species delineation. The major fatty acids (> 10%) were identified as C18:1 ω7c, C17:1 iso ω9c and C16:0. The main polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, phosphatidyl glycerol, unidentified lipid and unidentified aminolipid. Results from phenotypic, chemotaxonomic and genotypic analyses proposed that strain B2638T (= MCCC 1K07406T = KCTC 72968 T) is represented a novel species in the genus Novosphingobium, for which the names Novosphingobium beihaiensis sp. nov. is proposed.
摘要：
一种新的新颖性物种，指定菌株B2638T，是从北部湾收集的红树林沉积物中分离出来的，北海,P.R.中国。分离物可以在毒死rif的存在下生长。基于16SrRNA基因序列的系统发育分析显示，该分离株属于夜蛾属，与脱氯N.502str22T的序列相似性为99.9％，与该属的其他类型菌株的相似性小于98％。通过BOX-PCR进行分子分型，将菌株B2638T和去甲菌株502str22T分为两个簇，表明它们不相同。通过菌株B2638T及其近邻之间的DNA-DNA杂交（dDDH）和平均核苷酸同一性（ANI）值进行的基因组比较为20.0-29.5％和75.3-85.3％，分别,低于拟议的细菌种类划定阈值。主要脂肪酸(>10%)被鉴定为C18:1ω7c，C17:1异ω9c和C16:0。主要极性脂质含有二磷脂酰甘油，磷脂酰乙醇胺，鞘糖脂,磷脂酰甘油，身份不明的脂质和身份不明的氨基脂质。来自表型的结果，化学分类学和基因型分析提出，菌株B2638T（=MCCC1K07406T=KCTC72968T）是Novosphingoum属的新物种，为此命名为北海新鞘草sp。11月。是提议的。
