Abstract：
Members of the genus Novosphingobium were frequently isolated from polluted environments and possess great bioremediation potential. Here, three species, designated B2637T, B2580T and B1949T, were isolated from mangrove sediments and might represent novel species in the genus Novosphingobium based on a polyphasic taxonomy study. Phylogenomic analysis revealed that strains B2580T, B1949T and B2637T clustered with Novosphingobium naphthalenivorans NBRC 102051T, \'N. profundi\' F72 and N. decolorationis 502str22T, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between isolates and their closely related species were less than 94 and 54 %, respectively, all below the threshold of species discrimination. The sizes of the genomes of isolates B2580T, B2637T and B1949T ranged from 4.4 to 4.6 Mb, containing 63.3-66.4 % G+C content. Analysis of their genomic sequences identified genes related to pesticide degradation, heavy-metal resistance, nitrogen fixation, antibiotic resistance and sulphur metabolism, revealing the biotechnology potential of these isolates. Except for B2637T, B1949T and B2580T were able to grow in the presence of quinalphos. Results from these polyphasic taxonomic analyses support the affiliation of these strains to three novel species within the genus Novosphingobium, for which we propose the name Novosphingobium album sp. nov. B2580T (=KCTC 72967T=MCCC 1K04555T), Novosphingobium organovorum sp. nov. B1949T (=KCTC 92158T=MCCC 1K03763T) and Novosphingobium mangrovi sp. nov. B2637T (KCTC 72969T=MCCC 1K04460T).
摘要：
Novosphingobium属的成员经常从污染环境中分离出来，具有巨大的生物修复潜力。这里,三个物种,指定B2637T,B2580T和B1949T,从红树林沉积物中分离出来，根据多相分类学研究，可能代表了Novosphingobium属中的新物种。系统基因组分析显示，菌株B2580T，B1949T和B2637T聚集了萘新鞘胺NBRC102051T，\'N.profundi\'F72和N。脱色是502str22T，分别。分离株及其密切相关种之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别小于94％和54％，分别,都低于物种歧视的门槛。分离株B2580T的基因组大小，B2637T和B1949T的范围为4.4至4.6Mb，含有63.3-66.4%的G+C含量。分析他们的基因组序列，确定与农药降解相关的基因，耐重金属，固氮,抗生素抗性和硫代谢，揭示了这些分离株的生物技术潜力。除B2637T外,B1949T和B2580T能够在quinalphos存在下生长。这些多相分类学分析的结果支持这些菌株与Novosphingobium属中的三个新物种的隶属关系，为此，我们建议命名为Novosphingobium专辑sp。11月。B2580T(=KCTC72967T=MCCC1K04555T),有机夜蛾。11月。B1949T(=KCTC92158T=MCCC1K03763T)和红绿夜蛾。11月。B2637T(KCTC72969T=MCCC1K04460T)。
