背景:脱细胞同种异体神经移植物(ANA)已成功应用于桥接面神经缺损,干细胞移植可以增强再生效果。到目前为止,毛囊表皮神经c干细胞来源的雪旺氏细胞样细胞(EPI-NCSC-SCLCs)联合ANAs桥接面神经缺损的应用尚未见报道。
方法:通过功能和形态学检查检测载有绿色荧光蛋白(GFP)标记的EPI-NCSC-SCLCs(ANA细胞)的ANAs对桥接大鼠面神经干缺损(5毫米长)的影响,与自体移植物和ANA相比,分别。
结果:(1)EPI-NCSC-SCLC在体外与ANAs具有良好的相容性。(2)在ANA+细胞组中,小动物体内成像系统在8周内观察到GFP信号,术后16周在组织切片中检测到GFP标记的EPI-NCSC-SCLCs。(3)ANA+细胞组术后休息时面部对称性优于ANA组(p<0.05),与自体移植组相似(p>0.05)。ANA组的振动和眼睑运动的初始恢复时间比其他两组晚2周。(4)有髓纤维,ANA组颊支髓鞘厚度和轴突直径均显著差于其他两组(P<0.05),ANA+细胞组的结果与自体移植组的结果相似(p>0.05)。
结论:EPI-NCSC-SCLCs可促进大鼠面神经缺损的功能和形态恢复,GFP标记可以在体内追踪移植的EPI-NCSC-SCLCs一定时间。这些可能为周围神经缺损的临床治疗提供新的选择。
BACKGROUND: Acellular nerve allografts (ANAs) have been successfully applied to bridge facial nerve defects, and transplantation of stem cells may enhance the regenerative results. Up to now, application of hair follicle epidermal neural crest stem cell-derived Schwann cell-like cells (EPI-NCSC-SCLCs) combined with ANAs for bridging facial nerve defects has not been reported.
METHODS: The effect of ANAs laden with green fluorescent protein (GFP)-labeled EPI-NCSC-SCLCs (ANA + cells) on bridging rat facial nerve trunk defects (5-mm-long) was detected by functional and morphological examination, as compared with autografts and ANAs, respectively.
RESULTS: (1) EPI-NCSC-SCLCs had good compatibility with ANAs in vitro. (2) In the ANA + cells group, the GFP signals were observed by in vivo imaging system for small animals within 8 weeks, and GFP-labeled EPI-NCSC-SCLCs were detected in the tissue slices at 16 weeks postoperatively. (3) The facial symmetry at rest after surgery in the ANA + cells group was better than that in the ANA group (p < 0.05), and similar to that in the autograft group (p > 0.05). The initial recovery time of vibrissal and eyelid movement in the ANA group was 2 weeks later than that in the other two groups. (4) The myelinated fibers, myelin sheath thickness and diameter of the axons of the buccal branches in the ANA group were significantly worse than those in the other two groups (P < 0.05), and the results in the ANA + cells group were similar to those in the autograft group (p > 0.05).
CONCLUSIONS: EPI-NCSC-SCLCs could promote functional and morphological recovery of rat facial nerve defects, and GFP labeling could track the transplanted EPI-NCSC-SCLCs in vivo for a certain period of time. These may provide a novel choice for clinical treatment of peripheral nerve defects.