UASSIGNED:傅立叶重叠重叠显微镜(FPM)通过获取一系列低分辨率强度图像,可以实现具有大视场和高分辨率的定量相位成像,这些图像对应于在傅立叶域中拼接在一起的不同空间频率。然而,成像系统中各种像差的存在会显著降低重建结果的质量。现有的嵌入式光瞳函数恢复(EPRY-FPM)像差校正算法由于采用优化策略,成像性能和效率较低。
UNASSIGNED:提出了一种基于改进的相位恢复策略的像差校正方法(AA-P算法),以提高重建图像质量。
UNASSIGNED:此算法使用自适应调制因子,在更新迭代时添加这些函数,以优化样本的光谱函数和光学光瞳函数更新,分别。利用开源生物样本数据集进行仿真和实验,验证了该算法的有效性。
UNASSIGNED:实验结果表明,所提出的AA-P算法在具有混合像差的光学系统中,恢复复杂的振幅图像具有更清晰的轮廓和更高的相衬。与EPRY-FPM算法相比,图像重建质量提高了82.6%。
UNASSIGNED:提出的AA-P算法可以以更快的收敛速度重建更好的结果,恢复后的光瞳函数能更好地表征成像系统的像差。因此,我们的方法有望降低当前FPM对波前像差的严格要求。
Fourier ptychographic microscopy (FPM) enables quantitative phase imaging with a large field-of-view and high resolution by acquiring a series of low-resolution intensity images corresponding to different spatial frequencies stitched together in the Fourier domain. However, the presence of various aberrations in an imaging system can significantly degrade the quality of reconstruction results. The imaging performance and efficiency of the existing embedded optical pupil function recovery (EPRY-FPM) aberration correction algorithm are low due to the optimization strategy.
An aberration correction method (AA-P algorithm) based on an improved phase recovery strategy is proposed to improve the reconstruction image quality.
This algorithm uses adaptive modulation factors, which are added while updating iterations to optimize the spectral function and optical pupil function updates of the samples, respectively. The effectiveness of the proposed algorithm is verified through simulations and experiments using an open-source biological sample dataset.
Experimental results show that the proposed AA-P algorithm in an optical system with hybrid aberrations, recovered complex amplitude images with clearer contours and higher phase contrast. The image reconstruction quality was improved by 82.6% when compared with the EPRY-FPM algorithm.
The proposed AA-P algorithm can reconstruct better results with faster convergence, and the recovered optical pupil function can better characterize the aberration of the imaging system. Thus, our method is expected to reduce the strict requirements of wavefront aberration for the current FPM.