背景:开发安全有效的铁补充剂对于治疗缺铁性贫血很重要。因此,粗血红素蛋白提取物(CHPE)从亚洲鲈鱼鱼胆中提取没有(CON)和超声(US)辅助过程,然后冷冻干燥。测定所得冻干粗血红素蛋白提取物(FDCHPE)粉末的微量矿物质含量,颜色,二级结构,蛋白质模式,大小分布,挥发性化合物,和氨基酸组成。
结果:CON-FDCHPE和US-FDCHPE的提取率分别为6.76%和13.65%,分别。当施加70%振幅的US持续10分钟(US70/10)时,发现最高的血红素铁(0.485mg/mL)和非血红素铁(0.023mg/mL)含量。CON-FDCHPE和US-FDCHPE都没有重金属,但在US-FDCHPE中发现较高的铁含量(432.8mg/kg)(P<0.05)。在CON-FDCHPE和US-FDCHPE中观察到典型的红色,a*值分别为9.72和10.60。超声处理影响蛋白质结构,其中β-折叠激增,而随机线圈,α-螺旋,β转角减少。蛋白质模式证实两种样品都具有肌红蛋白作为主要蛋白质。US-FDCHPE还显示出更丰富的挥发性化合物,尤其是丙醛,己醛,庚醛,等等,与CON-FDCHPE相比。US-FDCHPE的氨基酸组成与联合国粮食及农业组织(FAO)的值相当。
结论:总体而言,使用超声处理提取的FDCHPE可以安全有效地强化食品中的铁补充剂,以缓解缺铁性贫血。此外,作为剩菜的g可以得到更好的利用,而不是被丢弃。©2023化学工业学会。
BACKGROUND: The development of a safe and effective iron supplement is important for the treatment of iron-deficient anemia. Therefore, the crude hemeprotein extract (CHPE) from Asian seabass gills was extracted without (CON) and with ultrasound (US)-assisted process, followed by freeze-drying. The resulting freeze-dried crude hemeprotein extract (FDCHPE) powders were determined for trace mineral content, color, secondary structure, protein pattern, size distribution, volatile compounds, and amino acid composition.
RESULTS: The extraction yields of CON-FDCHPE and US-FDCHPE were 6.76% and 13.65%, respectively. Highest heme iron (0.485 mg/mL) and non-heme iron (0.023 mg/mL) contents were found when US at 70% amplitude for 10 min (US 70/10) was applied. Both CON-FDCHPE and US-FDCHPE had no heavy metals, but higher iron content (432.8 mg/kg) was found in US-FDCHPE (P < 0.05). Typical red color was observed in CON-FDCHPE and US-FDCHPE with a*-values of 9.72 and 10.60, respectively. Ultrasonication affected protein structure, in which β-sheet upsurged, whereas random coil, α-helix, and β-turn were reduced. Protein pattern confirmed that both samples had myoglobin as the major protein. US-FDCHPE also showed a higher abundance of volatile compounds, especially propanal, hexanal, heptanal, and so forth, compared to CON-FDCHPE. Amino acid composition of US-FDCHPE was comparable to Food and Agriculture Organization of the United Nations (FAO) values.
CONCLUSIONS: Overall, FDCHPE extracted using ultrasonication could be safe and effective for fortification in food products as an iron supplement to alleviate iron-deficient anemia. Additionally, gills as leftovers could be better exploited rather than being disposed. © 2023 Society of Chemical Industry.