■蜱是重要的吸血体外寄生虫,可以传播各种病原体,对人类和牲畜的福祉构成重大威胁。大别山壁虱病毒(DBTV)于2015年在湖北省大别山山区的长毛壁虱中首次发现,中国。近年来,DBTV已在中国各个地区被发现,包括山东,浙江,辽宁,湖北,云南,和贵州省。然而,关于DBTV滴音传输的研究还很少。
■本研究利用小RNA测序(sRNA-seq)方法鉴定了从河北承德和河南永城采集的蜱中的蜱相关病毒,导致在河北发现了一种新的DBTV菌株。通过RNA-seq和Sanger测序获得了DBTV河北菌株的完整编码基因组。此外,首次在实验室检查了DBTV在长H.longicornis中的传输实验。
■在承德收集的新蜕皮的成虫H.longicornis蜱中检测到DBTV,河北省。此外,在从承德收集的未喂食若虫和长毛H.longicornis雌性中也检测到DBTV,阳性率分别为20%和56.25%,分别。获得了DBTV的完整编码基因组(OP682840和OP716696),系统发育分析表明,DBTV河北菌株与先前报道的DBTV菌株成簇。此外,这种病毒在充血的雌性中观察到,鸡蛋,和后代的幼虫。
■有必要扩大DBTV调查的范围,尤其是在中国北方。这项研究表明,DBTV可以从充血的雌性传播给下一代的幼虫。此外,从承德地区收集的未喂食若虫和成虫(从充血的若虫蜕化)中检测到DBTV,这表明H.longicornis是DBTV的潜在传播宿主和水库。然而,目前还缺乏对DBTV的分离和致病性的研究,强调需要进一步研究以减轻对动物和人类健康的潜在危害。
UNASSIGNED: Ticks are important blood-sucking ectoparasites that can transmit various pathogens, posing significant threats to the wellbeing of humans and livestock. Dabieshan tick virus (DBTV) was initially discovered in 2015 in Haemaphysalis longicornis ticks from the Dabieshan mountain region in Hubei Province,
China. In recent years, DBTV has been discovered in various regions of
China, including Shandong, Zhejiang, Liaoning, Hubei, Yunnan, and Guizhou Provinces. However, the researches on tick-borne transmission of DBTV are scarce.
UNASSIGNED: This study utilized the small RNA sequencing (sRNA-seq) method to identify tick-associated viruses in ticks collected from Chengde in Hebei Province and Yongcheng in Henan Province, leading to the discovery of a new DBTV strain in Hebei. The complete coding genome of DBTV Hebei strain was obtained through RNA-seq and Sanger sequencing. Furthermore, the transmission experiment of DBTV in H. longicornis was examined in laboratory for the first time.
UNASSIGNED: DBTV was detected in newly molted adult H. longicornis ticks collected in Chengde, Hebei Province. Additionally, DBTV was also detected in both unfed nymphs and engorged females of H. longicornis collected from Chengde, with a positive rate of 20% and 56.25%, respectively. The complete coding genome of DBTV (OP682840 and OP716696) were obtained, and phylogenetic analysis revealed that the DBTV Hebei strain clustered with previously reported DBTV strains. Furthermore, this virus was observed in engorged females, eggs, and larvae of the subsequent generation.
UNASSIGNED: It is necessary to expand the scope of DBTV investigation, particularly in northern
China. This study demonstrated that DBTV can be transmitted from engorged females to larvae of the next generation. Moreover, the detection of DBTV in unfed nymphs and adults (which moulted from engorged nymphs) collected from the filed of Chengde suggests that H. longicornis serves as a potential transmission host and reservoir for DBTV through transstadial and transovarial transmission. However, there remains a lack of research on the isolation and pathogenicity of DBTV, highlighting the need for further studies to mitigate potential harm to the health of animals and humans.