PDF(Pubmed)
Abstract：
Haemaphysalis longicornis is a common tick species that carries several pathogens. There are few reports on the influence of different hosts on the structure of midgut microflora in H. longicornis. In this study, midgut contents of fully engorged female H. longicornis were collected from the surface of tiger (Panthera tigris) and deer (Dama dama). The bacterial genomic DNA of each sample was extracted, and the V3-V4 regions of the bacterial 16S rRNA were sequenced using the Illumina NovaSeq sequencing. The diversity of the bacterial community of the fully engorged female H. longicornis on the surface of tiger was higher than that of deer. In total, 8 phyla and 73 genera of bacteria annotations were detected in the two groups. At the phylum level, the bacterial phyla common to the two groups were Proteobacteria, Firmicutes, and Actinobacteriota. At the genus level, there were 20 common bacterial genera, among which the relative abundances of Coxiella, Morganella, Diplorickettsia, and Acinetobacter were high. The Morganella species was further identified to be Morganella morganii. The alpha diversity index indicated that the bacterial diversity of the tiger group was higher than that of the deer group. Bacteroidota, Patescibacteria, Desulfobacterota, Verrucomicrobiota, and Cyanobacteria were solely detected in the tiger group. A total of 52 bacterial genera were unique in the tiger group, while one bacterial genus was unique in the deer group. This study indicates that there are differences in the structure of the gut bacteria of the same tick species among different hosts. Further culture-based methods are needed to provide a more comprehensive understanding of the tick microbiota parasitizing different hosts.
摘要：
长钩状芽孢杆菌是一种常见的蜱类，携带多种病原体。关于不同宿主对长尾H.longicornis中肠菌群结构的影响的报道很少。在这项研究中,从老虎（Pantheratigris）和鹿（Damadama）的表面收集了完全充血的雌性H.longicornis的中肠内容物。提取每个样品的细菌基因组DNA，和使用IlluminaNovaSeq测序对细菌16SrRNA的V3-V4区域进行测序。虎表面上完全膨胀的雌性H.longicornis的细菌群落多样性高于鹿。总的来说,注解在两组中均检测到8个门系和73个属的细菌。在门一级，两组共同的细菌门是变形杆菌，Firmicutes,和放线菌.在属一级，有20个常见的细菌属，其中柯西拉的相对丰富，Morganella,Diplorickettsia,和不动杆菌高。进一步鉴定了摩根氏菌物种为摩根氏菌。α多样性指数表示老虎群的细菌多样性高于鹿群。拟杆菌,芽孢杆菌,脱硫杆菌,Verrucomicrobiota,蓝细菌仅在老虎群中检测到。共有52个细菌属在老虎群中独一无二，而一个细菌属在鹿群中是独一无二的。这项研究表明，不同宿主之间相同蜱种的肠道细菌结构存在差异。需要进一步的基于培养的方法来提供对寄生于不同宿主的蜱微生物群的更全面的理解。
