目的:坏死梭杆菌引起牛肝脓肿,脚腐病,乳腺炎,还有子宫内膜炎.43kDa的外膜蛋白(43KOMP)是一种孔蛋白,在该细菌的感染中起重要作用,但是这种蛋白质的生物学功能和发病机理尚不清楚。
方法:在本研究中,我们通过串联质量标记蛋白质组学分析研究了43KOMP在牛乳腺上皮细胞(MAC-T细胞)细菌感染中的作用。将RAW264.7细胞与重组43KOMP(12.5μg/mL)孵育2小时,4h,6h,和12小时,然后通过Westernblot分析和ELISA检测炎症相关蛋白和炎症细胞因子的产生,实时定量PCR检测炎性细胞因子mRNA表达水平。
结果:蛋白质组学分析结果表明,与对照组相比,用43KOMP刺激的MAC-T细胞中有224种差异表达的蛋白质。118个蛋白质上调,106个蛋白质下调。这些差异表达蛋白主要参与NF-κB信号传导,细菌入侵上皮细胞,细胞粘附,补体和凝血级联。前六个差异表达蛋白是;MMP9,PLAU,斯托姆,PSMD13,PLAUR,还有ITGAV,参与蛋白质-蛋白质相互作用网络。此外,TLR/MyD88/NF-κB通路相关蛋白和炎性细胞因子(IL-6,TNF-α,和IL-1β)通过蛋白质印迹分析和ELISA进行评估。结果显示43KOMP在刺激后2h增强TLR4蛋白的表达(P<0.01),在4h增强MyD88蛋白的表达(P<0.05),并在4小时降低IκBα表达,感染后6h和12h(P<0.05),以及诱导Ser536的磷酸化(P<0.01)。IL-6,IL-1β,小鼠巨噬细胞上清液中的TNF-α升高(P<0.05),IL-6,IL-1β的mRNA表达水平,和TNF-α(P<0.05),IL-4mRNA表达降低(P<0.05)。
结论:综合来看,这些结果表明43KOMP在坏死F.感染中的重要作用,通过激活TLR/MyD88/NF-κB途径促进促炎细胞因子(IL-6和TNF-α)的产生。这些发现为更好地理解坏死F.crophorum感染的发病机制提供了理论依据。
OBJECTIVE: Fusobacterium necrophorum causes bovine hepatic abscess, foot rot, mastitis, and endometritis. The 43 kDa outer membrane protein (43 K OMP) of F. necrophorum is a porin protein that plays an important role in infections by this bacterium, but the biological function and the pathogenesis of this protein are largely unknown.
METHODS: In this study, we investigated the role of the 43 K OMP in bacterial infection of bovine mammary epithelial cells (MAC-T cells) by Tandem Mass Tag proteomic analysis. The RAW264.7 cells were incubated with recombinant 43 K OMP (12.5 μg/mL) for 2 h, 4 h, 6 h, and 12 h, and then the inflammatory related protein and inflammatory cytokine production were measured by Western blot analysis and ELISA, the mRNA expression levels of inflammatory cytokine were measured by Real-Time PCR.
RESULTS: Proteomic analysis results demonstrated there were 224 differentially expressed proteins in the MAC-T cells stimulated with the 43 K OMP compared with control, and 118 proteins were upregulated and 106 proteins were downregulated. These differentially expressed proteins were mainly involved in NF-kappa B signaling, bacterial invasion of epithelial cells, cell adhesion, complement and coagulation cascades. The top six differentially expressed proteins were; MMP9, PLAU, STOM, PSMD13, PLAUR, and ITGAV, which were involved in a protein-protein interaction network. Furthermore, TLR/MyD88/NF-κB pathway related proteins and inflammatory cytokines (IL-6, TNF-α, and IL-1β) were assessed by Western blot analysis and ELISA. Results showed the 43 K OMP to enhance the expression of TLR4 protein at 2 h (P < 0.01) and the MyD88 protein at 4 h (P < 0.05) post-stimulation, and to decrease IκBα expression at 4 h, 6 h and 12 h (P < 0.05) post-infection, as well as induce phosphorylation at Ser536 (P < 0.01). Levels of IL-6, IL-1β, and TNF-α in the supernatants of mouse macrophages were increased (P < 0.05), as were mRNA expression levels of IL-6, IL-1β, and TNF-α (P < 0.05), while IL-4 mRNA expression was decreased (P < 0.05).
CONCLUSIONS: Taken together, these results suggested the important role for 43 K OMP in F. necrophorum infection, promoting the production of pro-inflammatory cytokines (IL-6 and TNF-α) by activation of the TLR/MyD88/NF-κB pathway. These findings provided a theoretical basis for a better understanding of the pathogenesis of F. necrophorum infection.