中性粒细胞代表先天免疫的重要资产。中性粒细胞表达髓过氧化物酶(MPO),其是参与微生物杀灭的含血红素的过氧化物酶。在这项研究中,通过使用实时定量PCR和蛋白质印迹分析,比目鱼MPO(PoMPO)在头肾中高表达,接着是脾脏,吉尔,和肠道在个体发育期间-在从幼虫到成虫的发育阶段。此外,PoMPO阳性细胞存在于比目鱼各发育阶段的主要免疫器官中,随着鱼生长到幼年阶段,中性粒细胞的数量普遍较高。此外,流式细胞术分析显示,PoMPO阳性细胞相对于白细胞的比例,在腹膜腔中,头肾,比目鱼幼年期外周血占18.3%,34.8%,和6.0%,分别,这与先前报道的比目鱼的成年阶段相似。在个体发育过程中PoMPO的存在和组织分布表明,PoMPO阳性细胞确实在比目鱼的所有发育阶段都是先天免疫的参与者。
Neutrophils represent an important asset of innate immunity. Neutrophils express myeloperoxidase (MPO) which is a heme-containing peroxidase involved in microbial killing. In this study, by using real-time quantitative PCR and Western blot analysis, the
flounder MPO (PoMPO) was observed to be highly expressed in the head kidney, followed by spleen, gill, and intestine during ontogeny - during developmental stages from larvae to adults. Furthermore, PoMPO positive cells were present in major immune organs of
flounder at all developmental stages, and the number of neutrophils was generally higher as the fish grew to a juvenile stage. In addition, flow cytometry analysis revealed that the proportion of PoMPO positive cells relative to leukocytes, in the peritoneal cavity, head kidney, and peripheral blood of
flounder juvenile stage was 18.3 %, 34.8 %, and 6.0 %, respectively, which is similar to the adult stage in
flounder as previously reported. The presence and tissue distribution of PoMPO during ontogeny suggests that PoMPO positive cells are indeed a player of the innate immunity at all developmental stages of
flounder.