Abstract：
Annexin A2 (AnxA2), belonging to the annexin family, plays a crucial role in immune responses. In this study, the cDNA of the AnxA2 gene was identified in half-smooth tongue sole, Cynoglossus semilaevis. The transcript of AnxA2 gene in C. semilaevis (CsAnxA2) showed broad tissue distribution, with the highest expression level observed in the gut. CsAnxA2 expression was significantly up-regulated in the intestine, spleen, and kidney tissues following exposure to Shewanella algae. Immunohistochemical staining revealed that CsAnxA2 was predominantly expressed in epithelial cells and significantly elevated after S. algae challenge. Subcellular localization showed that CsAnxA2 was primarily localized in the cytoplasmic compartment. Moreover, proinflammatory cytokines (IL-6, IL-8 and IL-1β) exhibited significant upregulation after CsAnxA2 was overexpressed in vivo. One hundred and fifty-eight CsAnxA2-interacting proteins were captured in the intestinal tissue, showing the top two normalized abundance observed for actin beta (ACTB) and protein S100-A10 (p11). Fifty-four high abundance CsAnxA2-interacting proteins (HIPs) were primary enriched in ten pathways, with the top three significantly enriched pathways being Salmonella infection, glycolysis/gluconeogenesis, and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These results provide valuable information for further investigation into the functional mechanism of AnxA2 in C. semilaevis.
摘要：
膜联蛋白A2(AnxA2),属于阿尼辛家族,在免疫反应中起着至关重要的作用。在这项研究中,AnxA2基因的cDNA在半光滑舌下被鉴定,半舌肌。AnxA2基因在半乳杆菌(CsAnxA2)中的转录物呈广泛的组织分布,在肠道中观察到最高的表达水平。CsAnxA2表达在肠道中显著上调,脾,脾和暴露于希瓦氏菌藻类后的肾脏组织。免疫组织化学染色显示CsAnxA2主要在上皮细胞中表达,并且在S.藻类攻击后显著升高。亚细胞定位显示CsAnxA2主要位于细胞质区室中。此外，促炎细胞因子（IL-6，IL-8和IL-1β）在体内CsAnxA2过表达后显示出显着的上调。在肠组织中捕获了一百五十八个CsAnxA2相互作用蛋白，显示观察到的肌动蛋白β(ACTB)和蛋白S100-A10(p11)的前两个归一化丰度。54个高丰度的CsAnxA2相互作用蛋白（HIPs）主要富集在10个途径中，前三个显著丰富的途径是沙门氏菌感染，糖酵解/糖异生，和过氧化物酶体增殖物激活受体（PPAR）信号通路。这些结果为进一步研究AnxA2在半毛中的功能机制提供了有价值的信息。
