Abstract：
Circular RNAs (circRNAs) are non-coding RNAs with endogenous regulatory functions, including regulating skeletal muscle development. However, its role in the development of skeletal muscle in Japanese flounder (Paralichthys olivaceus) is not clear. Therefore we screened a candidate circpdlim5a, which is derived from the gene pdlim5a, from the skeletal muscle transcriptome of Japanese flounder. We characterized circpdlim5a, which was more stable compared to the linear RNA pdlim5a. Distributional characterization of circpdlim5a showed that circpdlim5a was predominantly distributed in the nucleus and was highly expressed in the skeletal muscle of adult Japanese flounder (24 months). When we further studied the circpdlim5a function, we found that it inhibited the expression of proliferation and differentiation genes according to the over-expression experiment of circpdlim5a in myoblasts. We concluded that circpdlim5a may inhibit the proliferation and differentiation of myoblasts and thereby inhibit skeletal muscle development in Japanese flounder. This experiment provides information for the study of circRNAs by identifying circpdlim5a and exploring its function, and offers clues for molecular breeding from an epigenetic perspective.
摘要：
环状RNA（circularRNAs）是具有内源性调节功能的非编码RNA，包括调节骨骼肌发育。然而,它在日本比目鱼（Paralichthysolivaceus）骨骼肌发育中的作用尚不清楚。因此，我们筛选了一个候选circpdlim5a，它来自基因pdlim5a，来自日本比目鱼的骨骼肌转录组。我们表征了circpdlim5a，与线性RNApdlim5a相比更稳定。cirpdlim5a的分布特征表明，cirpdlim5a主要分布在细胞核中，并在成年日本比目鱼（24个月）的骨骼肌中高度表达。当我们进一步研究circpdlim5a函数时，根据cirpdlim5a在成肌细胞中的过表达实验，我们发现它抑制了增殖和分化基因的表达。我们得出结论，cirpdlim5a可以抑制日本比目鱼成肌细胞的增殖和分化，从而抑制骨骼肌发育。本实验通过识别circpdlim5a和探索其功能为circRNAs的研究提供了信息，并从表观遗传学的角度为分子育种提供了线索。
