多种新出现的污染物的共存给生物体的生态生理功能带来了巨大的负担。联合毒性和潜在机制需要深入了解。这里,选择海洋蓝贻贝(MytilusgalloprovincialisL.)并暴露于2,2\',4,4'-四溴二苯醚(BDE-47)和全氟辛酸(PFOA)在环境相关浓度下单独和组合使用,以阐明应激反应和潜在毒理学机制的差异。积累的表征和比较,生物标志物,组织病理学,进行转录组学和代谢组学。共同暴露导致不同的积累模式,加剧了组织病理学改变,以及在氧化应激和生物标志物中的不同反应。此外,发现贻贝中鉴定的差异表达基因(DEG)和差异代谢产物(DEM)被注释为不同的代谢途径。相关性分析进一步表明,DEG和DEM与上述生物标志物显著相关。BDE-47和PFOA改变了与氨基酸代谢相关的基因和代谢产物,能量和嘌呤代谢,ABC运输商,和不同程度的谷胱甘肽代谢,随后诱导积累差异和联合毒性。此外,本工作强调了Nrf2-keap1解毒途径在galloprovincialis适应BDE-47和PFOA诱导的活性氧(ROS)胁迫中的关键作用。这项研究使人们能够更全面地了解多种新兴污染物污染的联合毒性机制。
The coexistence of multiple emerging contaminants imposes a substantial burden on the ecophysiological functions in organisms. The combined toxicity and underlying mechanism requires in-depth understanding. Here, marine blue mussel (Mytilus galloprovincialis L.) was selected and exposed to 2,2\',4,4\'-tetrabromodiphenyl ether (BDE-47) and perfluorooctanoic acid (PFOA) individually and in combination at environmental related concentrations to elucidate differences in stress responses and potential toxicological mechanisms. Characterization and comparison of accumulation, biomarkers, histopathology, transcriptomics and metabolomics were performed. Co-exposure resulted in differential accumulation patterns, exacerbated histopathological alterations, and different responses in oxidative stress and biomarkers for xenobiotic transportation. Moreover, the identified differentially expressed genes (DEGs) and differential metabolites (DEMs) in mussels were found to be annotated to different metabolic pathways. Correlation analyses further indicated that DEGs and DEMs were significantly correlated with the above biomarkers. BDE-47 and PFOA altered the genes and metabolites related to amino acid metabolism, energy and purine metabolism, ABC transporters, and glutathione metabolism to varying degrees, subsequently inducing accumulation differences and combined toxicity. Furthermore, the present work highlighted the pivotal role of Nrf2-keap1 detoxification pathway in the acclimation of M. galloprovincialis to reactive oxygen species (ROS) stress induced by BDE-47 and PFOA. This study enabled more comprehensive understanding of combined toxic mechanism of multi emerging contaminants pollution.