■糖尿病足溃疡(DFU)严重威胁患者的健康和生活质量。微生物群是DFU难治和高复发的主要原因。本研究旨在确定不同DFU阶段的伤口微生物群。
■从48例DFU患者中收集伤口样本,分为三个阶段:炎症(I,n=49),增殖(P,n=22),和重塑(R,n=19)。然后对在不同阶段获得的伤口样品进行16SrRNA基因测序。根据97%序列相似性的标准计算不同组中的操作分类单位(OTU)的数量。微生物群的多样性在门和属水平上差异呈现的细菌分类群,并进一步探讨了不同群体中重要的门和属。
■测序后,在I组中观察到3351、925和777个OTU,P,R,分别,和175个OTU重叠。与炎症阶段相比,创面微生物在增殖和重塑阶段的α-多样性显著降低(P<0.05)。在门一级,Firmicutes,变形杆菌,放线菌,拟杆菌是主要的门,占所有门的90%以上。在属一级,随机森林和线性判别分析效应大小分析表明,乳酸菌,普雷沃氏菌,Veillonella,Dialister,链球菌,和反刍动物是炎症阶段的标志性伤口微生物群;厌氧球菌,Ralstonia,放线菌,和Akkermansia是增殖阶段的重要物种;重塑阶段的关键属是肠杆菌,假单胞菌,Sondsrassella,双歧杆菌,和粪杆菌.
■不同阶段DFU患者伤口菌群的组成和结构存在显着差异,为有效促进DFU创面愈合奠定基础。
UNASSIGNED: Diabetic foot ulcers (DFU) seriously threaten the health and quality of life of patients. The microbiota is the primary reason for the refractory and high recurrence of DFU. This study aimed to determine the wound microbiota at different DFU stages.
UNASSIGNED: Wound samples were collected from 48 patients with DFU and divided into three phases: inflammatory (I, n = 49), proliferation (P, n = 22), and remodeling (R, n = 19). The wound samples obtained at different stages were then subjected to 16S rRNA gene sequencing. The number of operational taxonomic units (OTUs) in the different groups was calculated according to the criterion of 97 % sequence similarity. The diversity of the microbiota differentially presented bacterial taxa at the phylum and genus levels, and important phyla and genera in the different groups were further explored.
UNASSIGNED: After sequencing, 3351, 925, and 777 OTUs were observed in groups I, P, and R, respectively, and 175 OTUs overlapped. Compared with the inflammatory stage, the α-diversity of wound microbiota at proliferation and remodeling stages was significantly decreased (P < 0.05). At the phylum level, Firmicutes, Proteobacteria, Actinobacteriota, and Bacteroidota were the dominant phyla, accounting for more than 90 % of all the phyla. At the genus level, Random Forest and linear discriminant analysis effect size analyses showed that Peptoniphilus, Lactobacillus, Prevotella, Veillonella, Dialister, Streptococcus, and Ruminococcus were the signature wound microbiota for the inflammatory stage; Anaerococcus, Ralstonia, Actinomyces, and Akkermansia were important species for the proliferation stage; and the crucial genera for the remodeling stage were Enterobacter, Pseudomonas, Sondgrassella, Bifidobacterium, and Faecalibacterium.
UNASSIGNED: There were significant differences in the composition and structure of the wound microbiota in patients with DFU at different stages, which may lay a foundation for effectively promoting wound healing in DFU.