UNASSIGNED:帕金森病(PD)是一种常见的与年龄相关的慢性神经退行性疾病。目前没有负担得起的,有效,和较少侵入性的PD诊断测试。血液和基于血液的基因转录物中的代谢物谱分析被认为是诊断PD的理想方法。
未经批准:在这项研究中,目的是通过分析PD患者样本的微阵列基因表达数据,确定PD的潜在诊断生物标志物.
未经批准:一种计算方法,即,加权基因共表达网络分析(WGCNA)用于构建共表达基因网络,并从GSE99039数据集中鉴定与PD高度相关的关键模块。进行最小绝对收缩和选择算子(LASSO)回归分析以鉴定与PD强关联的关键模块中的中心基因。然后将选择的hub基因用于构建基于logistic回归分析的诊断模型,和受试者工作特征(ROC)曲线用于使用GSE99039数据集评估模型的功效。最后,使用逆转录聚合酶链反应(RT-PCR)来验证hub基因。
未经评估:WGCNA确定了与炎症和免疫反应相关的两个关键模块。七个枢纽基因,从两个模块中识别出LILRB1、LSP1、SIPA1、SLC15A3、MBOAT7、RNF24和TLE3,并用于构建诊断模型。ROC分析表明,该诊断模型在训练和测试数据集上对PD具有良好的诊断性能。RT-PCR实验结果表明,七个hub基因中LILRB1,LSP1和MBOAT7的mRNA表达存在显着差异。
未经证实:7基因组(LILRB1、LSP1、SIPA1、SLC15A3、MBOAT7、RNF24和TLE3)将作为PD的潜在诊断特征。
UNASSIGNED: Parkinson\'s disease (PD) is a common age-related chronic neurodegenerative disease. There is currently no affordable, effective, and less invasive test for PD diagnosis. Metabolite profiling in blood and blood-based gene transcripts is thought to be an ideal method for diagnosing PD.
UNASSIGNED: In this study, the objective is to identify the potential diagnostic biomarkers of PD by analyzing microarray gene expression data of samples from PD patients.
UNASSIGNED: A computational approach, namely, Weighted Gene Co-expression Network Analysis (WGCNA) was used to construct co-expression gene networks and identify the key modules that were highly correlated with PD from the GSE99039 dataset. The Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis was performed to identify the hub genes in the key modules with strong association with PD. The selected hub genes were then used to construct a diagnostic model based on logistic regression analysis, and the Receiver Operating Characteristic (ROC) curves were used to evaluate the efficacy of the model using the GSE99039 dataset. Finally, Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to validate the hub genes.
UNASSIGNED: WGCNA identified two key modules associated with inflammation and immune response. Seven hub genes, LILRB1, LSP1, SIPA1, SLC15A3, MBOAT7, RNF24, and TLE3 were identified from the two modules and used to construct diagnostic models. ROC analysis showed that the diagnostic model had a good diagnostic performance for PD in the training and testing datasets. Results of the RT-PCR experiments showed that there were significant differences in the mRNA expression of LILRB1, LSP1, and MBOAT7 among the seven hub genes.
UNASSIGNED: The 7-gene panel (LILRB1, LSP1, SIPA1, SLC15A3, MBOAT7, RNF24, and TLE3) will serve as a potential diagnostic signature for PD.