Characiformes是一种多样化且在进化上具有重要意义的淡水鱼,涵盖2,300多种物种。尽管多样性,由于基因组序列不足,我们对“异形”进化关系和适应机制的理解是有限的。在这项研究中,我们对四种Characiformes物种的基因组进行了测序和组装,其中三个是染色体水平的组装。我们的分析揭示了基因家族进化的动态变化,重复序列,以及这些基因组中染色体共线性的变化。有了组装的基因组,我们不仅能够阐明Otophysi中四个主要秩序的进化关系,但也表明Characiformes是近亲组。与其他可用鱼类基因组的比较基因组分析揭示了与Characiformes牙齿发育相关的基因的进化。值得注意的是,在Otophysi的不同顺序中观察到分泌性钙结合磷蛋白(SCPP)基因拷贝数的变化,表明它们对牙齿类型多样性的潜在贡献。我们的研究提供了宝贵的基因组序列和对Characiformes进化的新见解,为进一步的鱼类基因组和进化研究铺平了道路。
Characiformes is a diverse and evolutionarily significant order of freshwater fish encompassing over 2,300 species. Despite its diversity, our understanding of
Characiformes\' evolutionary relationships and adaptive mechanisms is limited due to insufficient genome sequences. In this study, we sequenced and assembled the genomes of four
Characiformes species, three of which were chromosome-level assemblies. Our analyses revealed dynamic changes in gene family evolution, repeat sequences and variations in chromosomal collinearity within these genomes. With the assembled genomes, we were not only able to elucidate the evolutionary relationship of the four main orders in Otophysi but also indicated
Characiformes as the paraphyletic group. Comparative genomic analysis with other available fish genomes shed light on the evolution of genes related to tooth development in Characiformes. Notably, variations in the copy number of secretory calcium-binding phosphoproteins (SCPP) genes were observed among different orders of Otophysi, indicating their potential contribution to the diversity of tooth types. Our study offers invaluable genome sequences and novel insights into
Characiformes\' evolution, paving the way for further genomic and evolutionary research in fish.