UV-C照射可以通过诱导果实抗病性和减少贮藏期间的腐烂来保持果实品质。将葡萄(VitisViniferaL.)暴露于2.4kJm-2UV-C照射下,然后接种碳曲霉,以研究营养品质的变化,防御相关物质和酶活性。采后UV-C照射可以增加防御相关物质和酶活性的水平,如酚类,黄烷醇,木质素,脯氨酸,谷胱甘肽,苯丙氨酸解氨酶(PAL),多酚氧化酶(PPO),和β-1,3-葡聚糖酶(GLU)。此外,通过基因表达水平的进一步验证,白藜芦醇在葡萄抗碳芽孢杆菌感染中发挥了重要作用。转录因子VvWRKY24和VvMYB14与VvSTS基因表达调控高度相关。本研究揭示了采后葡萄果实对UV-C辐射响应的分子机制和对黑腐病的防御机制,为采后葡萄贮藏保鲜技术提供理论依据。
UV-C irradiation can maintain fruit quality by inducing fruit disease resistance and reducing decay during storage. Grape (Vitis Vinifera L.) was exposed to 2.4 kJ m-2 UV-C irradiation then inoculated with Aspergillus carbonarius to investigate the changes in nutritional quality, defense related substances and enzyme activities. Postharvest UV-C irradiation can increased the levels of defense-related substances and enzyme activities, such as phenols, flavanols, lignin, proline, glutathione, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and β-1,3-glucanase (GLU). In addition, Resveratrol plays an important role in grape resistance to A. carbonarius infection through further verification by gene expression levels, the transcription factors VvWRKY24 and VvMYB14 are highly correlated with the regulation of VvSTS gene expression. This study revealed the molecular mechanism of postharvest grape fruit response to UV-C irradiation and the defense mechanism against black rot, and provided a theoretical basis for postharvest grape storage and preservation technology.