Abstract：
Copper selenide nanoparticles (CuSeNP) were synthesized using histidine, ethylenediamine, and sodium selenate as precursors by one-step microwave digestion methods. The as-prepared CuSeNPs exhibit excellent catechol oxidase mimic enzyme and catalase (CAT)-like activities. Dopamine (DA) can be oxidized to aminochrome with H2O2 by CuSeNPs, and the intermediate product aminochrome can further react with α-naphthol to yield a highly fluorescent derivative. It was confirmed that Cr(III) could adsorb on the surface of CuSeNPs and inhibit the production of semiquinone radicals in the reaction system, and the catalytic activity of CuSeNPs was inhibited. The detection mechanisms, kinetics, and catalytic properties of CuSeNPs were systematically investigated. As a result, a novel fluorescence method for the assay of Cr(III) was established. The feasibility of CuSeNP nanozyme in detecting speciation Cr(III) in food samples was explored with satisfactory results. It showed the obvious potential for developing effective and dependable fluorescent detection method for protecting food safety.
摘要：
利用组氨酸合成了硒化铜纳米粒子(CuSeNP),乙二胺,以硒酸钠为前驱体,采用一步微波消解法。制备的CuSeNP表现出优异的儿茶酚氧化酶模拟酶和过氧化氢酶（CAT）样活性。多巴胺（DA）可以被CuSeNP用H2O2氧化为氨基色素，和中间产物氨基色素可以进一步与α-萘酚反应以产生高度荧光的衍生物。证实了Cr(III)可以吸附在CuSeNPs表面,抑制反应体系中半醌自由基的产生,CuSeNPs的催化活性受到抑制。检测机制，动力学,系统研究了CuSeNPs的催化性能。因此,建立了测定Cr(Ⅲ)的荧光新方法。探索了CuSeNP纳米酶检测食品样品中形态Cr（III）的可行性，结果令人满意。它显示了开发有效可靠的荧光检测方法以保护食品安全的潜力。
