Mesh : Laccase / metabolism Basidiomycota / enzymology chemistry Mycelium / chemistry Microscopy, Electron, Scanning Catalase / metabolism Catechol Oxidase / metabolism Potassium / metabolism Chlorides / metabolism

来  源:   DOI:10.1615/IntJMedMushrooms.2024054258

Abstract:
To study and compare the morphology of the phellinoid Agaricomycetes strains and find other strategies to improve Phellinus spp. growth and metabolism. In this study, the morphological characteristics of four Phellinus igniarius strains (phellinoid Agaricomycetes) were observed under a light microscope. The exudates from these fungi were observed using light microscopy, scanning electron microscopy (SEM), and energy-dispersive spectrometry (EDS). The exudates were initially transparent with a water-like appearance, and became darker with time at neutral pH. Microscopy of air-dried exudates revealed regular shapes and crystals. Cl- (chloride) and K+ were the two key elements analyzed using EDS. Polyphenol oxidase (POD), catalase (CAT), and laccase activities were detected in mycelia from each of the four Phellinus strains. The K+ content of the three strains was higher than that of the wild strain. Cl- content correlated negatively with that of K+. Laccase activities associated with each mycelia and its corresponding media differed under cold and contaminated conditions.
摘要:
研究和比较类叶状蘑菇菌株的形态,并寻找其他改善桑黄的策略。生长和新陈代谢。在这项研究中,在光学显微镜下观察了4个桑黄菌株(类蘑菇)的形态特征。使用光学显微镜观察这些真菌的分泌物,扫描电子显微镜(SEM),和能量色散光谱法(EDS)。分泌物最初是透明的,外观像水一样,并且在中性pH下随时间变暗。风干渗出物的显微镜检查显示规则的形状和晶体。Cl-(氯化物)和K+是使用EDS分析的两个关键元素。多酚氧化酶(POD),过氧化氢酶(CAT),在四个桑黄菌株的菌丝体中检测到漆酶活性。三个菌株的K+含量均高于野生菌株。Cl-含量与K+含量呈负相关。在寒冷和污染条件下,与每个菌丝体及其相应培养基相关的漆酶活性不同。
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