YABBY基因家族是一种植物特异性转录因子,其DNA结合域参与多种功能,即风格调节,花的长度,开花植物侧生器官的极性发育。计算方法用于鉴定YABBY基因家族的成员,以胡萝卜(Daucuscarota)的基因组作为基础参考。基因的结构,染色体的位置,蛋白质基序和系统发育研究,综合征和转录组学分析,并对miRNA靶标进行了分析,以揭示胡萝卜中隐藏的YABBY基因家族的结构和功能特征。在接下来的研究中,已经得出结论,11个特定的YABBY基因不规则地分散在所有9条染色体和蛋白质上,组装成五个亚组,即AtINO,AtCRC,AtYAB5,AtAFO,和AtYAB2,它们是根据众所周知的拟南芥分类创建的。胡萝卜中广泛的YABBY基因由于节段复制而分散,当等同于串联重复时被检测为普遍存在。转录组分析表明,在胡萝卜主根的花色苷色素沉着过程中,DcYABBY基因之一高表达。顺式调控元件(CREs)分析揭示了对光特别有反应的元件,细胞周期调节,干旱诱导能力,ABA激素,种子,和分生组织表达。此外,在YABBY家族的胡萝卜和拟南芥基因中进行的一项相关研究表明,有5个亚家族具有共同的特征。基因组中YABBY基因的综合评价为克隆和理解其在胡萝卜中的功能特性提供了方向。我们的研究揭示了YABBY基因在胡萝卜中的全基因组分布和作用,与拟南芥最适合。
YABBY gene family is a plant-specific transcription factor with DNA binding domain involved in various functions i.e. regulation of style, length of flowers, and polarity development of lateral organs in flowering plants. Computational methods were utilized to identify members of the YABBY gene family, with
Carrot (Daucus carota) \'s genome as a foundational reference. The structure of genes, location of the chromosomes, protein motifs and phylogenetic investigation, syntony and transcriptomic analysis, and miRNA targets were analyzed to unmask the hidden structural and functional characteristics YABBY gene family in Carrots. In the following research, it has been concluded that 11 specific YABBY genes irregularly dispersed on all 9 chromosomes and proteins assembled into five subgroups i.e. AtINO, AtCRC, AtYAB5, AtAFO, and AtYAB2, which were created on the well-known classification of Arabidopsis. The wide ranges of YABBY genes in carrots were dispersed due to segmental duplication, which was detected as prevalent when equated to tandem duplication. Transcriptomic analysis showed that one of the DcYABBY genes was highly expressed during anthocyanin pigmentation in
carrot taproots. The cis-regulatory elements (CREs) analysis unveiled elements that particularly respond to light, cell cycle regulation, drought induce ability, ABA hormone, seed, and meristem expression. Furthermore, a relative study among
Carrot and Arabidopsis genes of the YABBY family indicated 5 sub-families sharing common characteristics. The comprehensive evaluation of YABBY genes in the genome provides a direction for the cloning and understanding of their functional properties in carrots. Our investigations revealed genome-wide distribution and role of YABBY genes in the carrots with best-fit comparison to Arabidopsis thaliana.