■藻蓝蛋白具有有利的生物学效应,包括免疫调节,抗癌,抗氧化剂,和抗炎能力。而PC,作为天然色素分子,不同于合成颜料,它可以在高温和光照条件下容易降解。
■在这项工作中,仔细研究了超声治疗对PC和间氯单宁复合物结构和功能特征的影响。通过UV-Vis研究了超声处理后PC与间氯单宁的相互作用,荧光光谱法,圆二色性(CD)光谱,傅里叶变换红外(FTIR)光谱。此外,评估了复合物的抗氧化潜力和体外消化率。
■结果表现为UV-Vis光谱降低效应,PC的CD光谱的荧光猝灭效应和弱构象变化。基于X-射线衍射(XRD)数据,PC被鉴定为无定形的,并且邻氯单宁被嵌入PC基质中。差示扫描量热法(DSC)结果表明,超声处理和添加间氯单宁可以将PC的变性峰温度(Td)提高到78.7°C。体外消化和自由基清除实验表明,适当的超声处理和添加间氯单宁对模拟胃肠道条件的抵抗力更强,可以提高DPPH和ABTS+自由基清除性能。
■超声处理和间氯单宁的添加改变了PC的结构和功能特性。这些结果证明了超声辅助从结节A中提取的间氯宁在改善PC功能特性方面的可行性,并为PC-多酚复合物作为功能性食品成分或生物活性材料的应用提供了可能性。
UNASSIGNED: Phycocyanin offers advantageous biological effects, including immune-regulatory, anticancer, antioxidant, and anti-inflammation capabilities. While PC, as a natural pigment molecule, is different from synthetic pigment, it can be easily degradable under high temperature and light conditions.
UNASSIGNED: In this work, the impact of ultrasound treatment on the complex of PC and phlorotannin structural and functional characteristics was carefully investigated. The interaction between PC and phlorotannin after ultrasound treatment was studied by UV-Vis, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, fourier transform infrared (FTIR) spectroscopy. Additionally, the antioxidant potential and in vitro digestibility of the complexes were assessed.
UNASSIGNED: The result was manifested as the UV-Vis spectrum reduction effect, fluorescence quenching effect and weak conformational change of the CD spectrum of PC. PC was identified as amorphous based on the X-ray diffraction (XRD) data and that phlorotannin was embedded into the PC matrix. The differential scanning calorimetry (DSC) results showed that ultrasound treatment and the addition of phlorotannin could improve the denaturation peak temperatures (Td) of PC to 78.7°C. In vitro digestion and free radical scavenging experiments showed that appropriate ultrasound treatment and the addition of phlorotannin were more resistant to simulated gastrointestinal conditions and could improve DPPH and ABTS+ free radical scavenging performance.
UNASSIGNED: Ultrasound treatment and the addition of phlorotannin changed the structural and functional properties of PC. These results demonstrated the feasibility of ultrasound-assisted phlorotannin from A. nodosum in improving the functional properties of PC and provided a possibility for the application of PC-polyphenol complexes as functional food ingredients or as bioactive materials.