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Abstract：
Polysaccharides from Ascophyllum nodosum (AnPS) were extracted and purified via an optimized protocol. The optimal extraction conditions were as follows: extraction time of 4.3 h, extraction temperature of 84 °C and ratio (v/w, mL/g) of extraction solvent (water) to raw material of 27. The resulting yield was 9.15 ± 0.23% of crude AnPS. Two fractions, named AnP1-1 and AnP2-1 with molecular weights of 165.92 KDa and 370.68 KDa, were separated from the crude AnPS by chromatography in DEAE Sepharose Fast Flow and Sephacryl S-300, respectively. AnP1-1 was composed of mannose, ribose, glucuronic acid, glucose and fucose, and AnP2-1 was composed of mannose, glucuronic acid, galactose and fucose. AnPS, AnP1-1 and AnP2-1 exhibited high scavenging activities against ABTS radical and superoxide radical, and showed protective effect on H₂O₂-induced oxidative injury in RAW264.7 cells. Furthermore, the immunostimulatory activities of AnP1-1 and AnP2-1 were evaluated by Caco-2 cells, the results showed both AnP1-1 and AnP2-1 could significantly promote the production of immune reactive molecules such as interleukin (IL)-8, IL-1β, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Therefore, the results suggest that AnPS and its two fractions may be explored as a potential functional food supplement.
摘要：
通过优化的方案提取和纯化来自夜蛾（AnPS）的多糖。最佳提取条件为:提取时间4.3h,提取温度为84°C，比例为(v/w，mL/g)的提取溶剂(水)对原料的27。得到的粗AnPS的产率为9.15±0.23%。两个分数，命名为AnP1-1和AnP2-1，分子量分别为165.92KDa和370.68KDa，分别在DEAE琼脂糖FastFlow和SephacrylS-300中通过色谱法从粗AnPS中分离。AnP1-1由甘露糖组成，核糖,葡萄糖醛酸，葡萄糖和岩藻糖，AnP2-1由甘露糖组成，葡萄糖醛酸，半乳糖和岩藻糖。AnPS,AnP1-1和AnP2-1对ABTS自由基和超氧自由基具有较高的清除活性,对H2O2诱导的RAW264.7细胞氧化损伤具有保护作用。此外，用Caco-2细胞评价AnP1-1和AnP2-1的免疫刺激活性,结果表明,AnP1-1和AnP2-1均能显著促进白细胞介素(IL)-8、IL-1β等免疫反应分子的产生,干扰素（IFN）-γ，和肿瘤坏死因子（TNF）-α。因此,结果表明，AnPS及其两个部分可以作为潜在的功能性食品补充剂进行探索。
