UNASSIGNED:肌动蛋白相关蛋白2/3复合物亚基5(ARPC5)是肌动蛋白相关蛋白2/3复合物的成员之一,在细胞迁移和侵袭中起重要作用。然而,对表达模式知之甚少,预后价值,以及ARPC5在泛癌症中的生物学功能。因此,我们以ARPC5为切入点,探索一种新的癌症预后和免疫学生物标志物.
UASSIGNED:公共数据库,包括TCGA,GTEx,UCEC,用于分析泛癌中ARPC5的表达。应用人蛋白图谱网站获取ARPC5在不同组织中的表达,细胞系,和单细胞类型。采用单因素Cox回归分析和Kaplan-Meier分析探讨ARPC5在多种癌症中的预后价值。采用Spearman相关性分析研究ARPC5表达与肿瘤微环境评分的相关性,免疫细胞浸润,免疫相关基因,TMB,MSI,RNA修饰基因,DNA甲基转移酶,和肿瘤干性。此外,qPCR,蛋白质印迹,并进行免疫组织化学检测ARPC5在HCC组织和细胞系中的差异表达。CCK8EdU,流式细胞术,伤口愈合试验,并进行了transwell测定以探索其在肿瘤增殖中的作用,凋亡,迁移,和肝癌细胞之间的侵袭。
UNASSIGNED:ARPC5表达在大多数癌症类型中上调,并且在KIRC中与不良预后显著相关,KIRP,LGG,LIHC。正常组织中ARPC5的mRNA表达呈低组织和细胞特异性,细胞系,和单细胞类型。ARPC5表达与肿瘤微环境评分呈正相关,免疫浸润细胞,免疫检查点相关基因在大多数癌症。STAD和BRCA中的ARPC5与TMB呈正相关,MSI,和新抗原。我们还发现ARPC5与m1A相关基因的表达有关,m5C相关基因,m6A相关基因,和DNA甲基转移酶。在实验分析中,我们发现ARPC5在HCC组织和HCC细胞中显著高表达。功能上,沉默ARPC5显著降低增殖,迁移,和肝癌细胞的侵袭能力。
UNASSIGNED:ARPC5表达影响多种肿瘤的预后,与肿瘤免疫浸润和免疫治疗密切相关。此外,ARPC5可能作为癌基因起作用并促进HCC的肿瘤进展。
UNASSIGNED: Actin-related protein 2/3 complex subunit 5 (ARPC5) is one of the members of actin-related protein 2/3 complex and plays an important role in cell migration and invasion. However, little is known about the expression pattern, prognosis value, and biological function of ARPC5 in pan-cancer. Thus, we focus on ARPC5 as cut point to explore a novel prognostic and immunological biomarker for cancers.
UNASSIGNED: The public databases, including TCGA, GTEx, and UCEC, were used to analyze ARPC5 expression in pan-cancer. The Human Protein Atlas website was applied to obtain the expression of ARPC5 in different tissues, cell lines, and single-cell types. Univariate Cox regression analysis and Kaplan-Meier analysis were used to explore the prognosis value of ARPC5 in various cancers. Spearman\'s correlation analysis was performed to investigate the association between ARPC5 expression and tumor microenvironment scores, immune cell infiltration, immune-related genes, TMB, MSI, RNA modification genes, DNA methyltransferases, and tumor stemness. Moreover, qPCR, Western blot, and immunohistochemistry were carried out to examine the differential expression of ARPC5 in HCC tissues and cell lines. CCK8, EdU, flow cytometry, wound-healing assays, and transwell assays were conducted to explore its role in tumor proliferation, apoptosis, migration, and invasion among HCC cells.
UNASSIGNED: ARPC5 expression was upregulated in most cancer types and significantly associated with worse prognosis in KIRC, KIRP, LGG, and LIHC. mRNA expression of ARPC5 showed low tissue and cell specificity in normal tissues, cell lines, and single-cell types. ARPC5 expression was positively correlated with the tumor microenvironment scores, immune infiltrating cells, immune checkpoint-related genes in most cancers. ARPC5 in STAD and BRCA was positively associated with TMB, MSI, and neoantigens. We also discovered that ARPC5 was correlated with the expression of m1A-related genes, m5C-related genes, m6A-related genes, and DNA methyltransferases. In experiment analyses, we found that ARPC5 was significantly highly expressed in HCC tissues and HCC cells. Functionally, silencing ARPC5 dramatically decreased proliferation, migration, and invasion ability of HCC cells.
UNASSIGNED: ARPC5 expression affects the prognosis of multiple tumors and is closely correlated to tumor immune infiltration and immunotherapy. Furthermore, ARPC5 may function as an oncogene and promote tumor progression in HCC.