在过去的二十年里，越来越多的人担心抗生素在水生环境中的存在。酚类抗生素,如氯霉素（CMP），常用于兽医和水产养殖领域治疗感染，经常在水生环境中被检测到，但是关于CMP对非目标水生生物的影响的生态毒性信息很少，多代研究很少被研究。在这里，我们量化了连续三代（P0，F1和F2）暴露于亚致死浓度（0、5、10、30、50、70和90mgL-1）的CMP的花梗的人口统计学反应。我们的结果表明，与对照组相比，高浓度的CMP会显著降低孵化时的预期寿命，生成时间,所有三代人口的净繁殖率和固有率都在增加，和花锥菌F1代中的后代比例。随着世代的增加，高浓度的CMP对孵化时的预期寿命和净繁殖率显示出增加的毒性作用，但是对生成时间的不规则负面影响，人口增长的内在速度，轮虫后代的比例。这些结果表明，有必要进行多代研究，以防止对抗生素对水生生态系统的影响的评估不足。
During the last two decades, there has been increasing concerns about the presence of antibiotics in aquatic environments. Phenicol antibiotics, such as chloramphenicol (CMP), commonly used in the veterinary and aquaculture fields to treat infections, have been often detected in aquatic environments, but scarce ecotoxicity information regarding the effects of CMP on non-target aquatic organisms is available, and multigenerational studies are seldom studied. Here we quantified the demographic responses of Brachionus calyciflorus exposed to sublethal concentrations (0, 5, 10, 30, 50, 70 and 90 mg L-1) of CMP for three successive generations (P0, F1, and F2). Our results showed that compared to the control, higher concentrations of CMP significantly decreased the life expectancy at hatching, generation time, net reproductive rate and intrinsic rate of population increase in all three generations, and the proportion of mictic offspring in the F1 generation of B. calyciflorus. With increasing generations, higher concentrations of CMP showed increased toxic effects on life expectancy at hatching and net reproductive rate, but irregular negative effects on generation time, intrinsic rate of population increase, and proportion of mictic offspring of the rotifers. These results indicate that multigenerational studies are necessary to prevent insufficient assessments of the impact of antibiotics in aquatic ecosystems.
