正确设计的噬菌体疗法是噬菌体疗法成功结果的基石。在这里，我们概述了开发符合相关质量和安全要求的噬菌体鸡尾酒的不同策略和步骤。它是基于经验的噬菌体治疗知识从一个多世纪的经验，最近进行的研究，和新兴技术。我们强调选择适当的噬菌体，并描述了一种改进的Appelmans\'方法，以改善鸡尾酒中单独和集体治疗性噬菌体的整体性能。我们介绍了该方法的两个版本，它们通过所采用的评估噬菌体活性和协同作用的技术彼此不同：通过Omnilog®系统测量细菌生长的光度评估与细菌呼吸。
Properly designed bacteriophage therapeutics are the cornerstone for a successful outcome of bacteriophage therapy. Here we present an overview of the different strategies and steps that can be taken to develop a bacteriophage cocktail that complies with relevant quality and safety requirements. It is based on empirical bacteriophage therapy knowledge from over a century of experience, more recently performed studies, and emerging technologies. We emphasize the selection of adequate bacteriophages and describe a modified Appelmans\' method to improve the overall performance of therapeutic bacteriophages individually and collectively in the cocktail. We present two versions of the method, which differ from each other by the employed techniques to evaluate phage activity and
synergy: photometric assessment of bacterial growth versus measurement of bacterial respiration via the Omnilog® system.
