背景:先前的研究表明,益生菌和益生菌可以增强铁的吸收。益生菌结合益生元(合生元),包括人乳寡糖(HiMO),通常添加到婴儿和后续配方(FUF)。这些添加是否会增强铁强化的商业配方奶粉中的铁吸收尚不确定。
目的:我们确定了添加以下物质的效果:1)合生元(低聚半乳糖(GOS)+罗伊利莫杆菌[L.reuteri]),或2)HiMO2'-岩藻糖基乳糖(2'FL)对铁强化的FUF对泰国幼儿铁吸收的影响。
方法:在随机分组中,控制,单盲(参与者)交叉研究,82名年龄在8-14个月的泰国儿童被招募食用单份(235ml)的FUF,并带有同位素标记的硫酸亚铁(2.2mg铁),其中:1)合生元(400mg/100mlGOS和罗伊氏乳杆菌DSM17938);2)2'FL(100mg/100ml);3)无合生元,无2'FL(对照),在3天之间通过在消耗最后一次测试FUF后14天(n=26)和28天(n=76)测量同位素标记的红细胞掺入来评估分数铁吸收(FIA[%])。
结果:铁强化FUF与合生元(8.2[5.2,12.9]%)和2'FL(8.4[5.5,14.1]%)的中位数(IQR)FIA与对照FUF(8.1[4.8,14.7]%)没有差异(合生元与control,P=0.24;2'FLvs.control,P=0.95)。在红细胞掺入14天和28天后测量时,来自所有FUF的FIA没有差异(时间,P=0.368;FUF,P=0.435;时间xFUF,P=0.937)。粪便pH和血红蛋白与FIA呈负相关。
结论:在年轻的泰国儿童中,添加合生元(GOS+L.reuteri)或2'FL到铁强化的FUF不会影响FIA。该试验在ClinicalTrial.gov注册为NCT04774016。该研究在clinicaltrials.gov注册为NCT04774016。
BACKGROUND: Previous studies showed that pre- and probiotics may enhance iron absorption. Probiotics combined with prebiotics (synbiotics), including human-identical milk oligosaccharides (HiMOs), are commonly added to infant and follow-up formula (FUF). Whether these additions enhance iron absorption from iron-fortified commercial milk formula is uncertain.
OBJECTIVE: We determined the effect of adding 1) a synbiotic [galacto-oligosaccharides [GOSs] + Limosilactobacillus reuteri (L. reuteri)] or 2) the HiMO 2\'-fucosyllactose (2\'FL) to iron-fortified FUF on iron absorption in young Thai children.
METHODS: In a randomized, controlled, single-blinded (participants) crossover
study, 82 Thai children aged 8-14 mo were enrolled to consume single servings (235 mL) of FUF with isotopically labeled ferrous sulfate (2.2 mg iron) with 1) the synbiotic (400 mg/100 mL GOS and L. reuteri DSM 17938), 2) the 2\'FL (100 mg/100 mL), and 3) without synbiotic and 2\'FL (control) in random order and a 3-d washout period between administrations. Fractional iron absorption [FIA (%)] was assessed by measuring erythrocyte incorporation of isotopic labels 14 d (n = 26) and 28 d (n = 76) after consumption of the last test FUF.
RESULTS: Median (IQR) FIA from iron-fortified FUF with the synbiotic [8.2 (5.2, 12.9)%] and with 2\'FL [8.4 (5.5, 14.1)%] did not differ from the control FUF [8.1 (4.8,14.7)%] (synbiotic compared with control, P = 0.24; 2\'FL compared with control, P = 0.95). FIA from all FUF did not differ when measured after 14 and 28 d of erythrocyte incorporation (Time, P = 0.368; FUF, P = 0.435; Time × FUF, P = 0.937). Fecal pH and hemoglobin were negatively associated with FIA.
CONCLUSIONS: In young Thai children, the addition of a synbiotic (GOS + L. reuteri) or 2\'FL to iron-fortified FUF did not impact FIA from a single serving. The
study was registered at clinicaltrials.gov as NCT04774016.