迄今为止,据报道,环孢菌属有20多种。其中,cayetanensiscyclosporacayetanensis已被认为是人类环孢菌素病的病原体,其特征是严重的肠道损伤和长期腹泻患者的免疫功能障碍。已经证实了牛中存在C.cayetanensis。迄今为止,然而,没有关于环孢菌的发生和患病率的监测数据.在山西省的牛,华北。在本研究中,从三个代表性县的牛身上采集的粪便样本共761份(齐,冀山,和山阴)在该省进行了环孢菌属的检查。通过使用基于核小亚基核糖体RNA(SSUrRNA)基因的聚合酶链反应限制性片段长度多态性(PCR-RFLP)测试。环孢菌属的患病率。在牛中占2.1%,和区域,年龄,性别,和品种未被确定为危险因素。基于SSUrRNA序列的分子进化分析显示,所有12个分离株都与人类病原体C.cayetanensis相对较远;七个分离株与Cyclosporacolobi分组,而其他人则与牛环孢菌属属属。以前报道过。虽然在本研究中没有在牛中检测到cayetanensis,应该在人群中进行更多的调查,其他动物种类,或从“一个健康”的角度来看,来自山西省其他地区的牛和其他环境来源。
To date, more than 20 species in the genus Cyclospora have been reported. Among them, Cyclospora cayetanensis has been recognized as the causative agent of human cyclosporiasis, which is characterized by severe intestinal injury and prolonged diarrhea in patients with immune dysfunction. The presence of C. cayetanensis in cattle has been confirmed. To date, however, no surveillance data are available on the occurrence and prevalence of Cyclospora spp. in cattle in Shanxi Province, North China. In the present study, a total of 761 fecal samples collected from cattle in three representative counties (Qi, Jishan, and Shanyin) in this Province were examined for Cyclospora spp. by using a polymerase-chain-reaction-restriction-fragment-length polymorphism (PCR-RFLP) test based on the nuclear small subunit ribosomal RNA (SSU rRNA) gene. The prevalence of Cyclospora spp. in cattle was 2.1%, and region, age, sex, and breed were not identified to be risk factors. Molecular evolutionary analysis based on the SSU rRNA sequences revealed that all 12 of the isolates were relatively distant from the human pathogen C. cayetanensis; seven isolates were grouped with Cyclospora colobi, whereas the others were grouped with cattle Cyclospora spp. reported previously. Though C. cayetanensis was not detected in cattle in the present study, more investigations should be performed in human populations, other animal species, or cattle from other regions of Shanxi Province and other environmental sources from the One Health perspective.