MicroRNAs(miRNA)与儿童急性淋巴细胞白血病(ALL)发病机制有关。我们对儿童ALL与健康儿童相比的miRNA单核苷酸多态性(SNPs)进行了系统评价和荟萃分析。这表明(i)pri-miR-34b/c中rs4938723的CC基因型和miR-100中rs543412的TT基因型赋予儿童针对ALL发生的保护作用;(ii)miR-146a中的rs2910164基因型与儿童ALL之间没有显着关联;(iii)DROSHA中的SNP,miR-449b,miR-938、miR-3117和miR-3689d-2基因似乎与儿童对B-ALL的易感性相关。对已发表的关于与对照组相比,ALL儿童中miRNAs差异表达的文献的综述揭示了miR-128家族的显着上调。miR-130b,miR-155,miR-181家族,miR-210、miR-222、miR-363和miR-708,以及miR-143和miR-148a的显著下调,似乎对儿童的所有发展都有明确的作用。儿童所有亚型中的microRNA特征,以及B-ALL和T-ALL病例之间的差异miRNA表达模式,被仔细检查。关于T-ALL儿科病例,我们用稳健而敏感的管道重新分析了RNA-seq数据集,并证实了hsa-miR-16-5p的显著差异表达,hsa-miR-19b-3p,hsa-miR-92a-2-5p,hsa-miR-128-3p(排名第一),hsa-miR-130b-3p和-5p,hsa-miR-181a-5p,-2-3p和-3p,hsa-miR-181b-5p和-3p,hsa-miR-145-5p和hsa-miR-574-3p,正如文献中所描述的,以及新鉴定的miRNA。
MicroRNAs (miRNAs) have been implicated in childhood acute lymphoblastic leukemia (ALL) pathogenesis. We performed a systematic
review and meta-analysis of miRNA single-nucleotide polymorphisms (SNPs) in childhood ALL compared with healthy children, which revealed (i) that the CC genotype of rs4938723 in pri-miR-34b/c and the TT genotype of rs543412 in miR-100 confer protection against ALL occurrence in children; (ii) no significant association between rs2910164 genotypes in miR-146a and childhood ALL; and (iii) SNPs in DROSHA, miR-449b, miR-938, miR-3117 and miR-3689d-2 genes seem to be associated with susceptibility to B-ALL in childhood. A
review of published literature on differential expression of miRNAs in children with ALL compared with controls revealed a significant upregulation of the miR-128 family, miR-130b, miR-155, miR-181 family, miR-210, miR-222, miR-363 and miR-708, along with significant downregulation of miR-143 and miR-148a, seem to have a definite role in childhood ALL development. MicroRNA signatures among childhood ALL subtypes, along with differential miRNA expression patterns between B-ALL and T-ALL cases, were scrutinized. With respect to T-ALL pediatric cases, we reanalyzed RNA-seq datasets with a robust and sensitive pipeline and confirmed the significant differential expression of hsa-miR-16-5p, hsa-miR-19b-3p, hsa-miR-92a-2-5p, hsa-miR-128-3p (ranked first), hsa-miR-130b-3p and -5p, hsa-miR-181a-5p, -2-3p and -3p, hsa-miR-181b-5p and -3p, hsa-miR-145-5p and hsa-miR-574-3p, as described in the literature, along with novel identified miRNAs.