PDF(Pubmed)
Abstract：
Lumpy skin disease (LSD) is a viral disease predominantly affecting cattle caused by a poxvirus belonging to the capripoxvirus genus. Here, we present a protocol for next-generation sequencing of the LSD virus genome using an amplicon-based approach. We describe steps for DNA extraction, viral DNA enrichment, amplicon pooling and purification, and library preparation and pooling. We then detail procedures for sequencing and computational analysis. This protocol can be adapted to any Illumina sequencing platform as an accelerated and scalable system. For complete details on the use and execution of this protocol, please refer to Bhatt et al.1,2.
摘要：
结块皮肤病(LSD)是一种病毒性疾病,主要影响牛,由属于羊痘病毒属的痘病毒引起。这里,我们提出了使用基于扩增子的方法对LSD病毒基因组进行下一代测序的方案.我们描述了DNA提取的步骤，病毒DNA富集，扩增子汇集和纯化，以及图书馆的准备和汇集。然后，我们详细介绍了测序和计算分析的程序。该方案可以适用于任何Illumina测序平台作为加速和可扩展的系统。有关此协议的使用和执行的完整详细信息，请参考Bhattetal.1,2。
