本研究的目的是研究火龙果果皮多酚提取物(DFPE)对产气量的影响,瘤胃发酵,和使用体外技术在梅花鹿中的细菌群落。不同DFPE水平的三种治疗方法(DFPE0,基础饮食;DFPE5,基础饮食+5mg/gDFPE;DFPE10,基础饮食+10mg/gDFPE,分别实施;n=6)。DFPE的酚类成分,天然气生产(GP),氨氮(NH3-N),挥发性脂肪酸(VFA),并在孵育24小时后评估细菌群落。结果表明,添加DFPE可降低GP和NH3-N。总VFA,异戊酸,和戊酸通过添加DFPE而增加(p<0.05)。pH值无变化(p>0.05),乙酸,丙酸,异丁酸,丁酸,和乙酸与丙酸的比例。此外,阿尔法指数,包括Sobs,香农,和Ace,通过DFPE补充增加。此外,在门的水平,DFPE补充增加(p=0.01)类细菌,但减少(p<0.01)Firmicutes。在属一级,与DFPE0相比,DFPE10增加了Rikenellaceae_RC9_gut_组的相对丰度(p<0.01),norank_f_Muribaculaceae(p=0.01),Lachnospiliaceae_NK3A20_组(p<0.01),Christensenellaceae_R-7_组(p<0.01),和NK4A214_组(p<0.01),链球菌的相对丰度降低(p<0.01),杆菌(p=0.01),和肠球菌(p<0.01)。与DFPE0相比,DFPE5在属水平的所有细菌中没有变化(p>0.05),除了肠球菌的相对丰度降低(p<0.01)。这些结果表明,DFPE可能能够用作饲料添加剂,以提高梅花鹿的发酵参数并改善瘤胃细菌群落。
The aim of this
study is to investigate the effect of dragon fruit peel polyphenolic extract (DFPE) on gas production, rumen fermentation, and bacterial communities in sika deer using an in vitro technique. Three treatments with different DFPE levels (DFPE0, base diet; DFPE5, base diet + 5 mg/g DFPE; DFPE10, base diet + 10 mg/g DFPE, respectively; n = 6) were implemented. The phenolic composition of DFPE, gas production (GP), ammonia nitrogen (NH3-N), volatile fatty acid (VFA), and bacteria communities was evaluated after 24 h of incubation. The results showed that GP and NH3-N were reduced by DFPE supplementation. Total VFA, isovaleric acid, and valeric acid were increased (p < 0.05) by the addition of DFPE. No changes (p > 0.05) were observed in pH, acetic acid, propionic acid, isobutyric acid, butyric acid, and the ratio of acetic acid to propionic acid. Additionally, the alpha indexes, including Sobs, Shannon, and Ace, were increased by DFPE supplementation. Moreover, at the phylum level, DFPE supplementation increased (p = 0.01) Bacteroidota but reduced (p < 0.01) Firmicutes. At the genus level, compared to DFPE0, the DFPE10 had increased relative abundances of Rikenellaceae_RC9_gut_group (p < 0.01), norank_f_Muribaculaceae (p = 0.01), Lachnospiraceae_NK3A20_group (p < 0.01), Christensenellaceae_R-7_group (p < 0.01), and NK4A214_group (p < 0.01), decreased relative abundances of Streptococcus (p < 0.01), Oribacterium (p = 0.01), and Enterococcus (p < 0.01). Compared to DFPE0, DFPE5 had no change (p > 0.05) in all bacteria at the genus level except for decreased relative abundance of Enterococcus (p < 0.01). These results indicated that DFPE may be able to be used as a feed additive to enhance fermentation parameters and improve ruminal bacteria communities in Sika deer.