To evaluate the effect of build direction, post-polymerization time, and aging on the fracture resistance, failure mode, margin quality and Marginal (MD) and Internal Discrepancies (ID), and degree of conversion of tridimensional (3D) printed provisional crowns using the liquid crystal display technique (LCD). A left mandibular first molar was prepared and scanned. After, a full crown was designed (ExoCad) and exported to the LCD-printer software. One hundred and eighty crowns were printed at two build directions (30°, 90°) and post-polymerized for different periods (15, 30, 45 min). Half of the samples were aged (ST) in distilled water (37 °C/90 days). The marginal quality of the crowns was evaluated by the Schriwer method under a stereomicroscope (40x). The replica technique was used to measure the MD and ID and measured under a stereomicroscope (10x), through 20 reference points defined in 5 regions: Occlusal (O), Cusp (CP), Axial (AX), Chamfer (CH), and Finish Line (F). After, the crowns were cemented onto the respective dies using temporary cement and submitted to compression test (ISO150, 1 mm/min, 100 kgf).The failure mode was classified by Burke\'s fracture mode. The degree of conversion was evaluated through Fourier Transform Infrared Spectroscopy. The data (μm) of MD and ID and fracture resistance (N) were subjected to ANOVA (3 factors) and Tukey\'s test (5%); Weibull analysis was also performed for fracture resistance data (N). For MD, ID and fracture resistance ANOVA revealed that all factors (P < .001) were significant. For aged groups, the crowns printed at 30° showed lower MD (Tukey). The O_90_30 min (172.13A μm) and O_90_15 min (170.20A μm) groups showed the highest ID values. Higher resistance values were observed for the 30_45 min (844.30A N), 30_30 min (835.35A N), and 90_30 min (820.62A N) groups (Tukey). In the margin analysis, 98.6% of the crowns printed at 30° showed smooth margins without defects. The most prevalent fracture mode (41.7%) was Burke type 5. The degree of conversion (DC) increased with increasing post-polymerization time and aging. Printing provisional crowns at 30° provided lower crown MD and ID, as well as higher fracture resistance. A post-polymerization time of 30 min resulted in higher crown fracture resistance, while aging reduced the resistance of the crowns.

UNASSIGNED: Chronic n-Hexane poisoning is prevalent among workers in small and micro printing industries in China. Despite this, there is limited research on occupational health risk assessment in these sectors. Conducting comprehensive risk assessments at key positions and proposing effective countermeasures are essential.
UNASSIGNED: Data were collected from 84 key positions across 32 small and micro-sized printing enterprises. Air samples were tested for n-Hexane exposure levels in accordance with Chinese standards. Five risk assessment models were employed: COSHH, EPA, MOM, ICMM, and Technical Guide GBZ/T 289-2017 of China. The consistency of results across these models was analyzed.
UNASSIGNED: Workers in 84 job positions were categorized into four exposure groups, with exposure to n-Hexane for 8-10 h daily, 5-6 days weekly. Most positions operated with low automation levels (96.9% in printing, 5.9% in oil blending, and 42.9% in pasting), while others were manual. Localized ventilation rates were notably low in oil blending (23.5%), cleaning (14.3%), and pasting (9.5%) groups. n-Hexane concentrations exceeded Chinese occupational limits in 15.6% of printing, 17.7% of oil blending, and 21.4% of cleaning groups. Risk assessment models identified over 60% of work groups as high risk. Significant differences (p < 0.05) were found among the seven risk assessment methods. Consistency analysis revealed moderate agreement between the Chinese synthesis index and exposure index methods (k = 0.571, p < 0.01).
UNASSIGNED: The Chinese synthesis and exposure index methods from Technical Guide GBZ/T 289-2017 are practical and reliable for assessing n-Hexane exposure risks in small and micro printing enterprises. Cleaning and printing roles were found to be at the highest risk for n-Hexane exposure. These findings provide valuable insights for targeted risk management strategies to protect workers\' health in the industry.

Nano/microfabrication is of fundamental importance both in scientific and industrial situations. There are, therefore, many attempts at realizing easier, quicker, and more precise fabrication of various structures; however, achieving this aim without a bulky and costly setup is still challenging. Here, we introduce a facile and versatile means of printing an ordered structure consisting of nanoscale stripes and more complicated geometries including pillars and wavy form with a lateral resolution of single micrometers. To this end, we prepare a polydimethylsiloxane (PDMS) slab with an oxygen plasma-induced wrinkled surface where liquid PDMS exudes by syneresis. Since this liquid PDMS is automatically loaded, the printing is repeatable without inking. A substrate moderately wettable to the liquid PDMS as well as amount/property-controlled syneresis is primarily important for the creation of well-defined structures. Precisely controlling these conditions will make this method universally applicable to diverse substrates and liquids including suspensions.

BACKGROUND: Long-term enophthalmos and diplopia resulting from orbital bone loss pose significant challenges in reconstructive surgery. This study evaluated the effectiveness of patient-specific porous titanium implants (PSIs) for addressing these conditions.
METHODS: This retrospective study involved 12 patients treated at Croix-Rousse Hospital, Lyon, from April 2015 to April 2022 who underwent late reconstruction via PSI for unilateral complex orbital bone loss. These implants were customized via 3D mirroring techniques on the basis of high-resolution computed tomography (CT) scans of the patients\' unaffected orbits.
RESULTS: All 12 patients presented with significant preoperative enophthalmos, with an average displacement of 3.24 mm, which was effectively corrected postoperatively to an average of 0.17 mm (p < 0.001). Orbital volume notably improved from a preoperative average of 3.38 mL to 0.37 mL postsurgery (p < 0.001). Functional improvements were evident as both enophthalmos and diplopia resolved completely. The Lancaster test revealed an improvement in the visual field, with 83.3 % of patients achieving normal results postoperatively.
CONCLUSIONS: By ensuring anatomical accuracy, patient-specific porous titanium implants, tailored from patient-specific imaging and fabricated via advanced 3D printing technology, provide a precise, effective, and reliable solution for reconstructing complex orbital defects and performing complicated revision surgeries.

Lateral flow assay (LFA) color signal quantification methods were developed by utilizing both International Commission on Illumination (CIE) LAB (CIELAB) color space and grayscale intensity differences. The CIELAB image processing procedure included calibration, test, control band detection, and color difference calculation, which can minimize the noise from the background. The LFA platform showcases its ability to accurately discern relevant colorimetric signals. The rising occurrence of infectious outbreaks from foodborne pathogens like Salmonella typhimurium presents significant economic, healthcare, and public health risks. The study introduces an aptamer-based lateral flow (ABLF) platform by using inkjet printing for specially detecting S. typhimurium. The ABLF utilized gold-decorated polystyrene microparticles, functionalized with specific S. typhimurium aptamers (Ps-AuNPs-ssDNA). The platform demonstrates a detection limit of 102 CFU mL-1 in buffer solutions and 103 CFU mL-1 in romaine lettuce tests. Furthermore, it sustained performance for over 8 weeks at room temperature. The ABLF platform and analysis methods are expected to effectively resolve the low-sensitivity problems of the former LFA systems and to bridge the gap between lab-scale platforms to market-ready solutions by offering a simple, cost-effective, and consistent approach to detecting foodborne pathogens in real samples.

UNASSIGNED: Biomechanical study.
UNASSIGNED: To investigate the mechanical characteristics of bone models created from medical images.
UNASSIGNED: Recent advancements in three-dimensional (3D) printing technology have affected its application in surgery. However, a notable gap exists in the analyses of how patient\'s dimorphism and variations in vertebral body anatomy influence the maximum insertional torque (MIT) and pullout strength (POS) of pedicle screws (PS) in osteoporotic vertebral bone models derived from medical images.
UNASSIGNED: Male and female patients with computed tomography data were selected. Dimensions of the first thoracic (T1), fourth lumbar (L4), and fifth lumbar (L5) vertebrae were measured, and bone models consisting of the cancellous and cortical bones made from polyurethane foam were created. PS with diameters of 4.5 mm, 5.5 mm, and 6.5 mm were used. T1 PS were 25 mm long, and L4 and L5 PS were 40 mm long. The bone models were secured with cement, and the MIT was measured using a calibrated torque wrench. After MIT testing, the PS head was attached to the machine\'s crosshead. POS was then calculated at a crosshead speed of 5 mm/min until failure.
UNASSIGNED: The L4 and L5 were notably larger in female bone models, whereas the T1 vertebra was larger in male bone models. Consequently, the MIT and POS for L4 and L5 were higher in female bone models across all PS diameters than in male bone models. Conversely, the MIT for T1 was higher in male bone models across all PS; however, no significant differences were observed in the POS values for T1 between sexes.
UNASSIGNED: The mechanical properties of the proposed bone models can vary based on the vertebral structure and size. For accurate 3D surgical and mechanical simulations in the creation of custom-made medical devices, bone models must be constructed from patientspecific medical images.

Printing as a process itself generates many environmental concerns. The paper addresses ink management in terms of environmental issues in the label printing industry, focusing on its environmental implications. The goal is to demonstrate how a proper ink management system impacts overall printing process efficiency and environmental sustainability for printing companies. The paper introduces an empirical approach to managing components for label and packaging production, utilizing automatic ink dispensing systems. The results demonstrate that the proper management of ink dispensing to minimize waste in packaging printing is crucial for optimizing operating print costs, potentially reducing the amount of ink needed to prepare colors by 52% and achieving energy savings of 37%. This approach fulfills the goal of sustainability by addressing environmental, economic, and social concerns. By optimizing ink usage and energy consumption, companies can significantly reduce operating costs and enhance economic performance. Simultaneously, these practices improve product quality, meet consumer demands for sustainable packaging, and create better working conditions for employees. Future directions and practical implications for supporting operational excellence in production are also discussed.

Molecular transport processes in printed polymer droplets hold enormous importance for understanding wetting phenomena and designing systems in applications such as encoding, electronics, photonics, and sensing. This paper studies thickness-dependent dewetting modes that are activated by thermal annealing and driven by interfacial interactions within microscopically confined polymeric features. The printing of poly(2-vinylpyridine) is performed in a regime where coffee-ring effects lead to strong thinning of the central region of the deposit. Thermal annealing leads to two different modes of dewetting that depend on the thickness of the central region. Mode I refers to the formation of randomly positioned small features surrounded by large hemispherical ones located along the periphery of the printed features and occurs when the central regions are thin. Observed at large central thicknesses, Mode II mediates significant molecular transport from edges toward the center of the printed droplet with thermal annealing and forms a hemispherical feature from the initial ring-like deposit. The selective adsorption of red, green, and blue emitting quantum dots over the poly(2-vinylpyridine) results in photoluminescent patterns. The selective assembly of photoluminescent quantum dots over patterned surfaces leads to deterministic and stochastic features beneficial to creating security labels for anti-counterfeiting applications.

UNASSIGNED: 3D-printing is a rapidly developing technology with applications in orthopaedics including pre-operative planning, intraoperative guides, design of patient specific instruments and prosthetics, and education. Existing literature demonstrates that in the surgical treatment of a wide range of orthopaedic pathology, using 3D printing shows favourable outcomes. Despite this evidence 3D printing is not routinely used in orthopaedic practice. We aim to evaluate the advantages of 3D printing in orthopaedic surgery to demonstrate its widespread applications throughout the field.
UNASSIGNED: We performed a comprehensive systematic review and meta-analysis. AMED, EMBASE, EMCARE, HMIC, PsycINFO, PubMed, BNI, CINAHL and Medline databases were searched using Healthcare Databases Advanced Search (HDAS) platform. The search was conducted to include papers published before 8th November 2020. Clinical trials, journal articles, Randomised Control Trials and Case Series were included across any area of orthopaedic surgery. The primary outcomes measured were operation time, blood loss, fluoroscopy time, bone fusion time and length of hospital stay.
UNASSIGNED: A total of 65 studies met the inclusion criteria and were reviewed, and 15 were suitable for the meta-analysis, producing a data set of 609 patients. The use of 3D printing in any of its recognised applications across orthopaedic surgery showed an overall reduction in operative time (SMD = -1.30; 95%CI: -1.73, -0.87), reduction in intraoperative blood loss (SMD = -1.58; 95%CI: -2.16, -1.00) and reduction in intraoperative fluoroscopy time (SMD = -1.86; 95%CI: -2.60, -1.12). There was no significant difference in length of hospital stay or in bone fusion time post-operatively.
UNASSIGNED: The use of 3D printing in orthopaedics leads to an improvement in primary outcome measures showing reduced operative time, intraoperative blood loss and number of times fluoroscopy is used. With its wide-reaching applications and as the technology improves, 3D printing could become a valuable addition to an orthopaedic surgeon\'s toolbox.

Single-cell analysis by mass spectrometry (MS) is emerging as a powerful tool that not only contributes to cellular heterogeneity but also offers an unprecedented opportunity to predict pathology onset and facilitates novel biomarker discovery. However, the development of single-cell MS analysis techniques with a focus on sample extraction, separation, and ionization methods for volume-limited samples and complexity of cellular samples are still a big challenge. In this study, we present a high-throughput approach to inkjet drop on demand printing single-cell MS for rapid screening of biomarkers of polycyclic aromatic hydrocarbon (PAH) exposure at the KYSE-150 cell, aiming to elucidate the pathogenesis of PAH-induced esophageal cancer. With an analytical bulk KYSE-150 cell throughput of up to 51 cells per minute, the method provides a new opportunity for simultaneous single-cell analysis of multiple biomarkers. We screened 930 characteristic ions from 3,683 detected peak signals and identified 91 distinctive molecules that exhibited significant differences under various concentrations of PAH exposure. These molecules have potential as clinical diagnostic biomarkers. Additionally, the current study identifies specific biomarkers that behave completely opposite in single-cell and multicell lipidomics as the concentration of PAH changes. These biomarkers potentially subdivide KYSE-150 cells into PAH-sensitive and PAH-insensitive types, providing a basis for revealing PAH toxicity and disease pathogenesis from the heterogeneity of cellular metabolism.