■研究表明,在动物实验中,间充质干细胞(MSCs)的移植可能是卵巢早衰(POF)的潜在候选星。然而,小样本量的个别研究不能用来得出明确的结论。因此,我们进行了系统评价和荟萃分析,以探讨使用MSCs治疗动物POF的潜力.
■搜索了七个数据库,用于探索在动物模型中MSCs移植对POF的影响的研究。遵循PRISMA准则,使用SYRCLE的偏倚风险工具确保了方法学质量。采用RevMan5.4和STATA12.0软件进行Meta分析。
■总共,包括涉及1,079只动物的37项研究。发现MSCs与E2水平显著相关(SMD2.69[95%CI1.97,3.41]),FSH(-2.02,[-2.74,-1.30]),初级卵泡(2.04,[1.17,2.92]),次级卵泡(1.93,[1.05,2.81]),和原始卵泡(2.38,[1.19,3.57]。其他成果,比如AMH,LH,INHB,窦卵泡,正在生长的卵泡,成熟卵泡,早期胃窦也被发现是重要的。FSH/LH无差异,黄体,卵泡,和estruc周期。
■我们的荟萃分析结果表明,MSCs移植可能对POF的动物模型产生治疗作用,这些影响可能与改善性周期的紊乱有关,调节血清激素表达到更好的状态,恢复卵巢功能.
UNASSIGNED: Studies have revealed that the transplantation of mesenchymal stem cells (MSCs) might be a potential star candidate for premature ovarian failure (POF) in animal experiments. However, individual studies with a small sample size cannot be used to draw a clear conclusion. Therefore, we conducted a systematic
review and meta-analysis to explore the potential of using MSCs in the treatment of POF in animals.
UNASSIGNED: Seven databases were searched for studies exploring the effect of the transplantation of MSCs on POF in animal models. The PRISMA guideline was followed, and the methodological quality was ensured using SYRCLE\'s risk of bias tool. RevMan 5.4 and STATA 12.0 software was performed to meta-analysis.
UNASSIGNED: In total, 37 studies involving 1,079 animals were included. Significant associations were found for MSCs with the levels of E2 (SMD 2.69 [95% CI 1.97, 3.41]), FSH (-2.02, [-2.74, -1.30]), primary follicles (2.04, [1.17, 2.92]), secondary follicles (1.93, [1.05, 2.81]), and primordial follicles (2.38, [1.19, 3.57]. Other outcomes, such as AMH, LH, INHB, antral follicles, growing follicles, mature follicles, and early antral were also found to be significant. There was no difference in FSH/LH, corpus leteum, follicles, and estruc cycle.
UNASSIGNED: Our meta-analysis result indicated that the transplantation of MSCs might exert therapeutic effects on animal models of POF, and these effects might be associated with improving the disorder of the sexual cycle, modulating serum hormone expressions to a better state, and restoring ovarian function.