背景:对基于血小板的疗法的兴趣日益增加,已经推动了新型兽医再生疗法的发展。血液衍生的富血小板纤维蛋白(PRF)包含大量的血小板和白细胞,高于生理基线,这些被认为是伤口再生的基本要素,刺激局部血管生成,细胞迁移,增殖和分化,被认为对皮肤修复至关重要。
目的:本研究旨在描述八只狗自然皮肤伤口的治疗方法,应用自体PRF治疗的地方,使用我们小组开发的协议。
方法:八只狗,年龄在7个月至9岁之间,来自不同的品种和性别,参加了这项研究。这些伤口中的四个被临床感染。在三种情况下,在治疗的第一周进行了两次PRF治疗,然后从第二周开始进行每周一次的治疗,直到出现外生肉芽组织。在每种情况下,只有当伤口完全闭合时,治疗才能完成.伤口没有接受局部防腐剂,抗生素或局部药物在治疗期间促进伤口愈合。
结果:PRF移植治疗在所有治疗的伤口中均有良好的耐受性,诱导显著的肉芽组织形成。PRF凝块充当天然组织填充物,促进上皮形成和伤口闭合,不需要局部使用抗菌/防腐剂,或额外的手术清创。在较大的损伤中记录到明显的皮肤收缩,并且所有的处理导致残留的美学疤痕,其中也观察到毛发生长。
结论:PRF疗法获得了有希望的结果,作为一种替代的伤口治疗,揭示了一种生物再生作用,促使自然的皮肤愈合过程。
The increasing interest in platelet-based therapies has underwritten the development of novel veterinary regenerative treatments. The haemoderivative platelet-rich fibrin (PRF) comprises abundant concentrations of platelets and leucocytes, above the physiologic baseline, which are considered essential elements for wound regeneration, stimulating local angiogenesis, cellular migration, proliferation and differentiation, considered essential for skin repair.
This study aimed to describe the treatment of eight dogs with naturally occurring cutaneous wounds, where autologous PRF therapy was applied, using a protocol developed by our group.
Eight dogs, aged between 7-month and 9-year old, from different breeds and sexes, were enrolled in this study. Four of these wounds were clinically infected. In three cases, two PRF treatments were performed during the first week of treatment, followed by single weekly treatments from the second week onwards, until exophytic granulation tissues were present. In each
case, the treatment was finalized only when complete wound closure was achieved. Wounds did not receive topical antiseptics, antibiotics or topical drugs to promote wound healing during the treatment.
PRF-grafting treatments were well tolerated in all treated wounds, inducing significant granulation tissue formation. PRF clots acted as a natural tissue filler, promoting epithelization and wound closure, without the requirement of topical antimicrobial/antiseptics application, or additional surgical debridement. Evident skin contraction was recorded in larger injuries and all the treatments resulted in vestigial aesthetic scars where hair growth was also observed.
PRF-therapy obtained promising results, as an alternative wound treatment, revealing a biological regenerative action, prompting the natural skin healing process.