DNA胞嘧啶甲基化,涉及基因调控和基因组稳定性的表观遗传机制,在不断变化的环境条件下,它的作用仍然知之甚少。先前在葡萄中使用甲基化敏感的扩增多态性(MSAP)标记的研究。Malbec克隆显示葡萄园特异性DNA甲基化多态性,但总体甲基化水平没有变化。为了补充这些发现,本研究通过移植实验研究了在不同葡萄园中种植的遗传相同植物之间的季节内表观遗传动态。同一克隆的插条,显示了由葡萄园(Agrelo和Gualtallary)施加的差异甲基化模式,在普通葡萄园(Lunlunta)中种植。采用高效液相色谱-紫外检测,全球DNA5-甲基胞嘧啶(5-mC)水平的定量显示葡萄中5-mC百分比相对较低,与Gualtalary植物(3.7%)相比,Agrelo中的含量更高(5.8%)。移植的植物保持了葡萄园之间的5-mC水平差异(9.8%vs6.2%),在随后的季节中持平(7.5%对7%)。此外,该研究使用MSAP标记在三个季节的Lununta移植植物中检查了5-mC多态性。在最初的生长季节,葡萄园在MSAP模式中观察到的差异逐渐减弱,表明在普通葡萄园中植入后对半甲基化模式进行了重新编程。相比之下,非甲基化图案表现出更高的稳定性,表明潜在的记忆效应。总的来说,这项研究提供了在不断变化的环境条件下葡萄DNA甲基化的动态性质的有价值的见解,对作物管理和育种策略有潜在影响。
DNA cytosine methylation, an epigenetic mechanism involved in gene regulation and genome stability, remains poorly understood in terms of its role under changing environmental conditions. Previous research using methylation-sensitive amplified polymorphism (MSAP) markers in a Vitis vinifera L. cv. Malbec clone showed vineyard-specific DNA methylation polymorphism, but no change in overall methylation levels. To complement these findings, the present
study investigates the intra-seasonal epigenetic dynamics between genetically identical plants grown in different vineyards through a transplanting experiment. Cuttings of the same clone, showing differential methylation patterns imposed by the vineyard of origin (Agrelo and Gualtallary), were cultivated in a common vineyard (Lunlunta). Using high-performance liquid chromatography-ultraviolet detection, the quantification of global DNA 5-methylcytosine (5-mC) levels revealed relatively low overall 5-mC percentages in grapevines, with higher levels in Agrelo (5.8%) compared to Gualtallary plants (3.7%). The transplanted plants maintained the 5-mC levels differences between vineyards (9.8% vs 6.2%), which equalized in subsequent seasons (7.5% vs 7%). Additionally, the
study examined 5-mC polymorphism using MSAP markers in Lunlunta transplanted plants over three seasons. The observed differences between vineyards in MSAP patterns during the initial growing season gradually diminished, suggesting a reprogramming of the hemimethylated pattern following implantation in the common vineyard. In contrast, the non-methylated pattern exhibited greater stability, indicating a potential memory effect. Overall, this
study provides valuable insights into the dynamic nature of DNA methylation in grapevines under changing environmental conditions, with potential implications for crop management and breeding strategies.