中链脂肪酸(MCFA)是具有6至12个碳原子的链长的膳食组分。MCFA可以穿过血脑屏障,在大脑中可以通过线粒体β-氧化被氧化。作为生酮饮食的成分,MCFA已证明对不同的脑疾病有益的影响,比如创伤性脑损伤,老年痴呆症,耐药癫痫,糖尿病,和癌症。尽管人们对MCFA效应感兴趣,关于大脑中MCFA代谢的信息不多。在这项研究中,使用基于气相色谱-质谱(GC-MS)的代谢组学方法,再加上多元数据分析,我们跟踪添加辛酸(C8)后U87MG胶质母细胞瘤细胞的代谢变化,或癸酸(C10)24小时。我们的分析强调了添加C8或C10后U87MG细胞代谢的显着差异,并确定了两组处理细胞之间数量变化的几种代谢物。总的来说,代谢途径分析表明柠檬酸循环,Warburg效应,谷氨酰胺/谷氨酸代谢,和酮体代谢作为受C8或C10添加到U87MG细胞影响的途径。我们的数据表明,C8影响线粒体代谢,导致酮体产生增加,C10主要通过刺激脂肪酸合成影响细胞溶质途径。此外,谷氨酰胺可能是支持C10处理细胞中脂肪酸合成的主要底物。总之,我们确定了与U87MG细胞中添加C8或C10相关的代谢特征,该特征可用于破译成胶质细胞瘤细胞对MCFA治疗的代谢反应.
Medium-chain fatty acids (MCFA) are dietary components with a chain length ranging from 6 to 12 carbon atoms. MCFA can cross the blood-brain barrier and in the brain can be oxidized through mitochondrial β-oxidation. As components of ketogenic diets, MCFA have demonstrated beneficial effects on different brain diseases, such as traumatic brain injury, Alzheimer\'s disease, drug-resistant epilepsy, diabetes, and cancer. Despite the interest in MCFA effects, not much information is available about MCFA metabolism in the brain. In this
study, with a gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach, coupled with multivariate data analyses, we followed the metabolic changes of U87MG glioblastoma cells after the addition of octanoic (C8), or decanoic (C10) acids for 24 h. Our analysis highlighted significant differences in the metabolism of U87MG cells after the addition of C8 or C10 and identified several metabolites whose amount changed between the two groups of treated cells. Overall, metabolic pathway analyses suggested the citric acid cycle, Warburg effect, glutamine/glutamate metabolism, and ketone body metabolism as pathways influenced by C8 or C10 addition to U87MG cells. Our data demonstrated that, while C8 affected mitochondrial metabolism resulting in increased ketone body production, C10 mainly influenced cytosolic pathways by stimulating fatty acid synthesis. Moreover, glutamine might be the main substrate to support fatty acids synthesis in C10-treated cells. In conclusion, we identified a metabolic signature associated with C8 or C10 addition to U87MG cells that can be used to decipher metabolic responses of glioblastoma cells to MCFA treatment.