Abstract：
Pokemon, a transcriptional repressor, inhibits the transcription of tumor suppressor genes and promotes tumor formation by affecting chromatin recombination or binding directly to the tumor suppressor genes. In this study, the mechanism behind the Pokemon regulation of liver cancer cell metabolism was evaluated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. Pokemon-overexpressing HL7702 cells were obtained by lipofection. The cells were collected at different time points after transfection and their intracellular metabolites were analyzed using an LC-MS-based metabolomic method. Based on the results of a multivariate statistical analysis, candidate metabolites with significant differences were selected, and 36 of them were confirmed by database and MS/MS analysis. These metabolites were primarily involved in lipid synthesis by KEGG database searches. Further analysis indicated that both the acetyl-coenzyme carboxylase and the fatty acid synthase were activated in the lipid synthesis pathway. The results show that Pokemon can affect cell metabolism by activating the lipid synthesis pathways in cells.
摘要：
口袋妖怪，转录抑制因子，抑制肿瘤抑制基因的转录，并通过影响染色质重组或直接与肿瘤抑制基因结合来促进肿瘤形成。在这项研究中,使用基于液相色谱-质谱(LC-MS)的代谢组学评估了Pokemon调节肝癌细胞代谢的机制.通过脂转染获得过表达口袋妖怪的HL7702细胞。在转染后的不同时间点收集细胞,并使用基于LC-MS的代谢组学方法分析其细胞内代谢物。根据多元统计分析的结果，选择具有显著差异的候选代谢物，其中36例通过数据库和MS/MS分析证实。通过KEGG数据库搜索，这些代谢物主要参与脂质合成。进一步的分析表明，乙酰辅酶羧化酶和脂肪酸合酶在脂质合成途径中均被激活。结果表明，口袋妖怪可以通过激活细胞中的脂质合成途径来影响细胞代谢。
