■免疫球蛋白G(IgG)在介导体液免疫和维持肠粘膜中的免疫稳态方面很重要。牛至精油(OEO)是一种天然的草药提取物,具有抗菌,抗氧化剂,抗炎,和免疫调节特性。由于OEO对荷斯坦奶牛肠道粘膜免疫的影响尚不清楚,我们调查了日粮补充OEO对荷斯坦奶牛肠道IgG水平和IgG+细胞的影响.
■选择12只健康约10个月大的荷斯坦公牛进行实验,并随机平均分为两组。对照组(CK)饲喂基础日粮,在OEO组,基础日粮补充OEO(20g/头/天)。经过300天的喂养,空肠组织样本,回肠,收集各组公牛的结肠进行组织病理学分析,免疫组织化学,和酶联免疫吸附测定,分别。
■空肠,回肠,CK组牛结肠有明显的病理损伤,而每个肠段的结构清晰完整。在OEO组中,病理损伤明显减轻。IgG+浆细胞弥散分布在空肠固有层中,回肠,CK和OEO组的结肠,组间无显著差异。OEO补充显着减少了每个肠段中IgG浆细胞的数量,回肠下降率最高(22.87%),其次是结肠(19.45%)和空肠(8.52%)。ELISA试验成果与免疫组化成果互相验证。IgG含量的变更与IgG+浆细胞数目的变更趋向相符。
■我们的研究结果表明,补充OEO不会改变荷斯坦奶牛肠道中IgG+浆细胞的扩散空间分布,但是将免疫球蛋白水平降低到正常水平,显著减少肠道损伤,并可能通过抑制炎症反应增强粘膜免疫防御屏障功能。
UNASSIGNED: Immunoglobulin G (IgG) is important in mediating humoral immunity and in the maintenance of immune homeostasis in the intestinal mucosa. Oregano essential oil (OEO) is a natural herbal extract that possesses antimicrobial, antioxidant, anti-inflammatory, and immunomodulatory properties. As the effects of OEO on intestinal mucosal immunity in Holstein dairy bulls remained unclear, we investigated the effect of dietary supplementation of OEO on IgG levels and IgG+ cells residing in the intestinal tract in Holstein dairy bulls.
UNASSIGNED: Twelve Holstein bulls in good health of approximately 10 months of age were selected for the experiment and randomly equally divided into two groups. The control (CK) group was fed a basal ration, and in the OEO group, the basal ration was supplemented with OEO (20 g/head/day). After 300 days of feeding, tissue samples of the
jejunum, ileum, and colon of the bulls in each group were collected for histopathological analysis, immunohistochemistry, and enzyme-linked immunosorbent assays, respectively.
UNASSIGNED: The
jejunum, ileum, and colon of bulls in the CK group had obvious pathological damage, whereas the structure of each intestinal segment was clear and intact. In the OEO group, pathological damage was significantly reduced. IgG+ plasma cells were diffusely distributed in the lamina propria of the
jejunum, ileum, and colon in the CK and OEO groups, with no significant difference between the groups. OEO supplementation significantly reduced the number of IgG+ plasma cells in each intestinal segment, with the highest decrease rate being noted for the ileum (22.87%), followed by the colon (19.45%) and
jejunum (8.52%). ELISA test results and immunohistochemical results were mutually verified. The change in IgG content was consistent with the trend of change in the number of IgG+ plasma cells.
UNASSIGNED: Our findings suggest that OEO supplementation does not alter the diffuse spatial distribution of IgG+ plasma cells in the intestines of Holstein dairy bulls, but lowers immunoglobulin levels to normal levels, significantly reduces intestinal damage, and may enhance mucosal immune defence barrier function by inhibiting inflammatory reactions.