背景:尽管各种产品通常用于嫩肤,作为可注射形式的固体型透明质酸(HA)尚未被研究或利用。本研究旨在证明螺纹形式的固体型HA的安全性和有效性。这不同于通常使用的常规凝胶型HA。
方法:固体型HA螺纹,常规HA填料,将聚二恶烷酮(PDO)线插入小鼠的背侧皮下层。在第0、1、3和7天拍摄照片,并且在第7天收获样品用于组织学分析。通过组织染色证实炎症反应和胶原蛋白的检测,并进行实时PCR以定量胶原合成。
结果:在组织学分析中,与HA线相比,PDO线表现出更大的炎症反应。Masson三色染色显示,与HA填充剂组相比,HA线组的胶原蛋白合成程度更高。PDO线组1型胶原表达明显高于HA线组,HA线组3型胶原表达水平较高。此外,与HA组相比,PDO线组TGF-β1在统计学上显著增加.
结论:这项体内研究证明了固体型纯HA线的稳定应用及其诱导胶原蛋白产生的潜力,同时也产生低炎症反应。这些发现突出了固体型HA在化妆品皮肤病学领域的应用前景。
方法:本期刊要求作者为每个提交的证据分配一个级别,该级别的证据适用于循证医学排名。这不包括评论文章,书评,和有关基础科学的手稿,动物研究,尸体研究,和实验研究。对于这些循证医学评级的完整描述,请参阅目录或在线作者说明www。springer.com/00266.
BACKGROUND: Although various products are commonly used for skin rejuvenation, solid-type hyaluronic acid (HA) as an injectable form has not been researched or utilized. This
study aimed to demonstrate the safety and efficacy of solid-type HA in thread form, which differs from the conventional gel-type HA commonly used.
METHODS: Solid-type HA threads, conventional HA fillers, and polydioxanone (PDO) threads were inserted into the dorsal subcutaneous layer of mice. Photographs were taken on days 0, 1, 3, and 7, and on day 7, the samples were harvested for histological analysis. Inflammatory reactions and detection of collagen were confirmed through tissue staining, and real-time PCR was conducted to quantify collagen synthesis.
RESULTS: In the histological analysis, the PDO threads exhibited a greater inflammatory response compared to the HA threads. Masson\'s trichrome staining revealed a higher degree of collagen synthesis in the HA thread group compared to the HA filler group. While collagen type 1 expression was significantly higher in the PDO thread group than in the HA thread group, the HA thread group showed higher expression levels of collagen type 3. Furthermore, the PDO thread group demonstrated a statistically significant increase in TGF-β1 compared to the HA group.
CONCLUSIONS: This in vivo
study demonstrated the stable application of solid-type pure HA threads and their potential for inducing collagen production, while also yielding a low inflammatory response. The findings highlight the promising applications of solid-type HA in the field of cosmetic dermatology.
METHODS: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .