副溶血性弧菌可引起人海产品传播的胃肠炎感染,甚至可导致死亡。副溶血弧菌的致病菌株分泌不同类型的毒力因子,这些毒力因子通过不同类型的分泌系统直接注射到宿主细胞中,这有助于细菌在生物体内建立自己的生态位。因此,这项研究的目的是从副溶血性弧菌的trh阳性菌株中分离细胞外分泌蛋白,并使用二维凝胶电泳和MALDI-TOFMS/MS进行鉴定。17种不同的细胞蛋白质,氨基甲酰磷酸合成酶,5-甲基四氢蝶酰三谷氨酸,tRNA-二氢尿苷合成酶,甘油-3-磷酸脱氢酶,泊苷5'-磷酸脱羧酶,钼进口ATP结合蛋白,DnaJ,DNA聚合酶IV,核糖体RNA小亚基甲基转移酶G,ATP合酶亚基δ和γ,核糖体回收因子,4-羟基-3-甲基丁-2-烯-1-基二磷酸合酶,tRNA假尿苷合成酶B,迪特兰,聚顺式-十一烯基二磷酸合成酶,氧依赖性的卟啉原-III氧化酶,和肽脱甲酰基酶2被鉴定为主要参与不同的代谢和生物合成途径。此外,鉴定的蛋白质的分子功能与催化活性有关,连接酶活性,运输机,金属装订,和ATP合成酶,当他们在细胞间。然而,为了了解这些分泌蛋白在宿主细胞内细菌感染和存活中的重要性,进行了病原体-宿主蛋白-蛋白相互作用(PPI),该相互作用鉴定了8种分泌蛋白与参与不同细胞途径的41种人类蛋白的关联。包括泛素化降解,附着力,炎症,豁免权,和程序性细胞死亡。本研究为成功建立感染和细胞内繁殖提供了有关宿主细胞环境生存和适应机制的未报道策略。
Vibrio parahaemolyticus causes seafood-borne gastroenteritis infection in human which can even lead to death. The pathogenic strain of V. parahaemolyticus secretes different types of virulence factors that are directly injected into the host cell by a different type of secretion system which helps bacteria to establish its own ecological niche within the organism. Therefore, the aim of this
study was to isolate the extracellular secreted proteins from the trh positive strain of V. parahaemolyticus and identify them using two-dimensional gel electrophoresis and MALDI-TOFMS/MS. Seventeen different cellular proteins viz, Carbamoyl-phosphate synthase, 5-methyltetrahydropteroyltriglutamate, tRNA-dihydrouridine synthase, Glycerol-3-phosphate dehydrogenase, Orotidine 5\'-phosphate decarboxylase, Molybdenum import ATP-binding protein, DnaJ, DNA polymerase IV, Ribosomal RNA small subunit methyltransferase G, ATP synthase subunit delta and gamma, Ribosome-recycling factor, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, tRNA pseudouridine synthase B, Ditrans, polycis-undecaprenyl-diphosphate synthase, Oxygen-dependent coproporphyrinogen-III oxidase, and Peptide deformylase 2 were identified which are mainly involved in different metabolic and biosynthetic pathways. Furthermore, the molecular function of the identified proteins were associated with catalytic activity, ligase activity, transporter, metal binding, and ATP synthase when they are intercellular. However, to understand the importance of these secreted proteins in the infection and survival of bacteria inside the host cell, pathogen-host protein-protein interactions (PPIs) were carried out which identified the association of eight secreted proteins with 41 human proteins involved in different cellular pathways, including ubiquitination degradation, adhesion, inflammation, immunity, and programmed cell death. The present
study provides unreported strategies on host-cell environment\'s survival and adaptation mechanisms for the successful establishment of infections and intracellular propagation.