C2 photosynthesis is a photosynthetic pathway in which photorespiratory CO2 release and refixation are enhanced in leaf bundle sheath (BS) tissues. The evolution of C2 photosynthesis has been hypothesized to be a major step in the origin of C4 photosynthesis, highlighting the importance of studying C2 evolution. In this study, physiological, anatomical, ultrastructural, and immunohistochemical properties of leaf photosynthetic tissues were investigated in six non-C4 Tribulus species and four C4 Tribulus species. At 42°C, T. cristatus exhibited a photosynthetic CO2 compensation point in the absence of respiration (C*) of 21 µmol mol-1, below the C3 mean C* of 73 µmol mol-1. Tribulus astrocarpus had a C* value at 42°C of 55 µmol mol-1, intermediate between the C3 species and the C2 T. cristatus. Glycine decarboxylase (GDC) allocation to BS tissues was associated with lower C*. Tribulus cristatus and T. astrocarpus allocated 86% and 30% of their GDC to the BS tissues, respectively, well above the C3 mean of 11%. Tribulus astrocarpus thus exhibits a weaker C2 (termed sub-C2) phenotype. Increased allocation of mitochondria to the BS and decreased length-to-width ratios of BS cells, were present in non-C4 species, indicating a potential role in C2 and C4 evolution.

There are great concerns for the accumulation in the environment of small dimension plastics, such as micro- and nanoplastics. Due to their small size, which facilitates their uptake by organisms, nanoplastics are of particular concern. The toxic effects of nanoplastics on plants are already reported in the literature, however nothing is known, to date, about the possible effects of climate change, in particular of increasing temperatures, on their toxicity for plants. To address this issue, plants of the water fern Azolla filiculoides were grown at optimal (25 °C) or high (35 °C) temperature, with or without polystyrene nanoplastics, and the effects of these stressors were assessed using a multidisciplinary approach. Green fluorescent polystyrene nanoplastics were used to track their possible uptake by A. filiculoides. The development and physiology of our model plant was adversely affected by both nanoplastics and high temperatures. Overall, histological, morphological, and photosynthetic parameters worsened under co-treatment, in accordance with the increased uptake of nanoplastics under higher temperature, as observed by fluorescence images. Based on our findings, the concern regarding the potential for increased toxicity of pollutants, specifically nanoplastics, at high temperatures is well-founded and warrants attention as a potential negative consequence of climate change. Additionally, there is cause for concern regarding the increase in nanoplastic uptake at high temperatures, particularly if this phenomenon extends to food and feed crops, which could lead to greater entry into the food chain.

BACKGROUND: Violent conflict is a formidable global challenge, with long-lasting impacts on individual health and society security. There has been compelling evidence that heat can increase aggression intention on the individual level. However, little is known about the short-term relationship between ambient temperature and collective violent conflicts, especially in less developed regions.
METHODS: We conducted a time-stratified case-crossover study combined with the distributed lag nonlinear model (DLNM) among 247,773 violent conflicts from 29 countries or regions in the Greater Middle East, between 1997 and 2021. Potential modification effects of economic status and climate conditions were explored by stratified analyses. Negative control and sensitivity analyses were also performed to test the robustness of our model.
RESULTS: We observed significant associations between higher temperature and the onset of five categories of violent conflicts. The effects generally occurred within the first several days after exposure. The incidence risks of battles, violence against civilians, explosions/remote violence, protests and riots were 1.60 [95 % confidence interval (CI): 1.31-1.95], 1.82 (95 % CI: 1.37-2.42), 1.24 (95 % CI: 1.08-1.41), 1.16 (95 % CI: 1.09-1.24) and 1.54 (95 % CI: 1.22-1.95) when comparing extreme high temperatures to minimum-risk temperatures. The associations were generally more prominent in areas with lower economic levels and associations in regions of the continental climate are also stronger.
CONCLUSIONS: Our finding reveals novel and concrete evidence that short-term high temperature could increase the risk of multiple forms of violent conflict in the Greater Middle East and provides new insights into the potential short-term mechanisms under the heat-collective violence association.

With high temperature becoming an increasing health risk due to a changing climate, it is important to quantify the scale of the problem. However, estimating the burden of disease (BoD) attributable to high temperature can be challenging due to differences in risk patterns across geographical regions and data accessibility issues.
We present a methodological framework that uses Köppen-Geiger climate zones to refine exposure levels and quantifies the difference between the burden observed due to high temperatures and what would have been observed if the population had been exposed to the theoretical minimum risk exposure distribution (TMRED). Our proposed method aligned with the Australian Burden of Disease Study and included two parts: (i) estimation of the population attributable fractions (PAF); and then (ii) estimation of the BoD attributable to high temperature. We use suicide and self-inflicted injuries in Australia as an example, with most frequent temperatures (MFTs) as the minimum risk exposure threshold (TMRED).
Our proposed framework to estimate the attributable BoD accounts for the importance of geographical variations of risk estimates between climate zones, and can be modified and adapted to other diseases and contexts that may be affected by high temperatures.
As the heat-related BoD may continue to increase in the future, this method is useful in estimating burdens across climate zones. This work may have important implications for preventive health measures, by enhancing the reproducibility and transparency of BoD research.

The aim of the study is to evaluate the association between summer temperatures and emergency department visits (EDVs) in Bologna (Italy) and assess whether this association varies across areas with different socioeconomic and microclimatic characteristics. We included all EDVs within Bologna residences during the summers of 2010-2019. Each subject is attributed a deprivation and a microclimatic discomfort index according to the residence. A time-stratified case-crossover design was conducted to estimate the risk of EDV associated with temperature and the effect modification of deprivation and microclimatic characteristics. In addition, a spatial analysis of data aggregated at the census block level was conducted by applying a Poisson and a geographically weighted Poisson regression model. For each unit increase in temperature above 26 °C, the risk of EDV increases by 0.4% (95%CI: 0.05-0.8). The temperature-EDV relationship is not modified by the microclimatic discomfort index but rather by the deprivation index. The spatial analysis shows that the EDV rate increases with deprivation homogeneously, while it diminishes with increases in median income and microclimatic discomfort, with differences across areas. In conclusion, in Bologna, the EDV risk associated with high temperatures is not very relevant overall, but it tends to increase in areas with a low socioeconomic level.

This short paper describes several experimental approaches for dealing with chemical reactions or decomposition which can occur when making thermophysical property measurements at high temperature and high pressure. The associated equipment was designed and built to allow thermophysical property data to be cast in a more realistic perspective by taking explicit account of chemical changes which may occur during an experiment. As an example of these methods, the measurements on the methanol system are discussed in detail.

Identifying the most vulnerable subjects is crucial for the effectiveness of health interventions aimed at limiting the adverse consequences of high temperatures. We conducted a case crossover study aimed at assessing whether suffering from mental health disorders modifies the effect of high temperatures on mortality. We included all deaths occurred in the area of Bologna Local Health Trust during the summers 2004-2017. Subjects with mental disorders were identified by using the local Mental Health Registry. A conditional logistic model was applied, and a z-test was used to study the effect modification. Several models were estimated stratifying by subjects\' characteristics. For every 1 °C above 24 °C, mortality among people without mental disorders increased by 1.9% (95% CI 1.0-2.6, p < 0.0001), while among mental health service users, mortality increased by 5.5% (95% CI 2.4-8.6, p < 0.0001) (z-test equal to p = 0.0259). The effect modification varied according to gender, residency and cause of death. The highest probability of dying due to an increase in temperature was registered in patients with depression and cognitive decline. In order to reduce the effects of high temperatures on mortality, health intervention strategies should include mental health patients among the most vulnerable subjects taking account of their demographic and clinical characteristics.

Sanitation workers are at high-risk of suffering from heat-related illnesses in high temperature weather. However, some well-known heat stress indexes have limitations in practical application. This paper aims to establish a new index-comprehensive physiological index (CPI) for evaluating physiological safety.
Firstly, the real-time weights of physiological parameters are obtained by entropy method. Secondly, the new index- CPI is established based on the distance between the measured values and the ideal solutions by the technique for order of preference by similarity to ideal solution (TOPSIS). Then the reasonability of CPI is verified. Finally, the safety range of the CPI is determined.
The mean skin temperature (MST), systolic blood pressure (SBP) and diastolic blood pressure (DBP) of the sanitation workers are greatly affected by the high temperature. The weight range of the MST, tympanic temperature, SBP, DBP and heart rate is 0.155-0.274, 0.146-0.200, 0.173-0.399, 0.150-0.298 and 0.146-0.200, respectively. And the weights of MST, SBP and DBP account for a relatively large proportion, ranging from 60.1% to 70.9%. The danger range of the CPI is (0, 0.6] while the safety range of the CPI is (0.6, 1.0].
The CPI can provide simple and easily-measured real-time monitoring of the physiological status. It has the potential to be a practical index for guaranteeing occupational health for sanitation workers in summer.

Although injury is a leading cause of death worldwide, the association between ambient temperature and injury has received little research attention compared to the association of temperature with mortality and morbidity from non-external causes. With current climate change and increases in weather extremes, assessing the association between temperature and injury is important for determining public health priorities. Therefore, the present study examined the association between ambient temperature and injury risk with a focus on the intentions and mechanisms of injury. Using the national emergency database, we identified a total of 703,503 injured patients who had visited emergency departments in Seoul, South Korea from 2008 to 2016. We conducted a time-stratified case-crossover study using a conditional Poisson regression model, and applied a distributed lag nonlinear model to explore possible nonlinear and delayed effects of daily mean temperature on injury risk. Injury risk was significantly associated with ambient temperature, and temperature-injury association curves markedly differed with respect to intentions and mechanisms of injury. Although unintentional injuries increased significantly at both high and low temperatures, intentional injuries - including self-harm and assault - significantly increased only at high temperatures. The mechanism-specific analyses showed that injuries caused by traffic accidents and burns significantly increased at both high and low temperatures. However, injuries caused by all other mechanisms (i.e., fall, blunt object, machinery, penetration, and poisoning) significantly increased only at high temperatures, while injury due to slipping increased at low temperatures. Our study provides evidence that ambient temperature is associated with risk of injury, and this association differs depending on the intentions and mechanisms of injury. Overall, our findings help foster a more comprehensive understanding of the association between temperature and injury that can be used to establish appropriate public health policies and targeted interventions.

A complex algorithm is proposed for the forensic medical service response in emergency situations marked by a large number of deaths from fire. Its use will promote increased efficiency in the work of forensic medical units while limiting the consequences to human victims in emergency situations.
Предложен комплексный алгоритм действий судебно-медицинской службы при чрезвычайных ситуациях (пожар), сопровождающихся массовой гибелью людей. Такой алгоритм позволит повысить эффективность работы судебно-медицинских формирований при ликвидации последствий чрезвычайных ситуаций с человеческими жертвами.