hCG

HCG
  • 文章类型: Journal Article
    青少年血管纤维瘤(JA)是一种罕见的,性别特异性,和高度血管化的鼻腔肿瘤,几乎只影响男性青少年,但其病因一直存在争议。G蛋白偶联激素受体LHCGR[黄体生成素(LH)/绒毛膜促性腺激素(hCG)受体]代表了阐明性别特异性潜在机制的有希望的新候选者。青春期表现,和JA进步。我们使用了高度敏感的RNAscope技术,连同免疫组织化学,为了研究细胞表达,本地化,和LHCGR在JA患者组织样本中的分布。我们的结果为整个JA组织切片的细胞亚群中LHCGR表达提供了证据,大多数LHCGR+细胞位于血管附近,使他们对内分泌LH/hCG信号敏感,但在纤维胶原间质中也检测到LHCGR+细胞。位于血管腔附近的大多数LHCGR+细胞共表达神经c干细胞标志物CD271。这些结果很有趣,因为LH和hCG都是以时间和性别依赖的方式产生的,并且已知能够诱导细胞增殖和血管生成。我们的结果产生了一个新的模型,表明涉及LHCGR及其配体的内分泌机制,与自分泌和旁分泌信号一起,在JA血管化和细胞增殖中。
    Juvenile angiofibroma (JA) is a rare, sex-specific, and highly vascularized nasal tumor that almost exclusively affects male adolescents, but its etiology has been controversial. The G protein-coupled hormone receptor LHCGR [luteinizing hormone (LH)/choriogonadotropin (hCG) receptor] represents a promising new candidate for elucidating the underlying mechanisms of sex specificity, pubertal manifestation, and JA progression. We used highly sensitive RNAscope technology, together with immunohistochemistry, to investigate the cellular expression, localization, and distribution of LHCGR in tissue samples from JA patients. Our results provide evidence for LHCGR expression in subsets of cells throughout JA tissue sections, with the majority of LHCGR+ cells located in close vicinity to blood vessels, rendering them susceptible to endocrine LH/hCG signaling, but LHCGR+ cells were also detected in fibrocollagenous stroma. A majority of LHCGR+ cells located near the vascular lumen co-expressed the neural crest stem cell marker CD271. These results are intriguing as both LH and hCG are produced in a time- and sex-dependent manner, and are known to be capable of inducing cell proliferation and angiogenesis. Our results give rise to a new model that suggests endocrine mechanisms involving LHCGR and its ligands, together with autocrine and paracrine signaling, in JA vascularization and cell proliferation.
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  • 文章类型: Journal Article
    我们的目标是评估在移植体外产生的(IVP)牛肉胚胎时使用人绒毛膜促性腺激素(hCG)治疗对泌乳多胎母牛泽西岛妊娠结局的影响。1级,7期(扩大胚泡),IVP牛肉胚胎是使用3个被证明具有高生育能力的Angussires从黑色的Angus大坝中产生的,并被冷冻以直接转移。在初步实验中,泌乳多胎泽西岛母牛被随机分配到2x2阶乘排列的处理中,以测试受体同步方案的主要影响(Double-Ovsynch;DO;n=169vs.同步发情;ED;n=180),并在接受者方案中随机分配作为未治疗的对照(DO-CON,n=78;ED-CON,n=44)或接受2,500IUhCG(DO-hCG,n=79;ED-hCG,n=46)在胚胎移植时(ET)。DO(93%)的受体利用率高于ED(50%)的母牛,受体同步方案和hCG治疗之间存在相互作用,其中DO-hCG母牛在26、33和61d时的每次胚胎移植妊娠(P/ET)比DO-CON多,ED-hCG,和ED-CON奶牛。根据部分预算分析,DO奶牛的每次怀孕费用比ED奶牛低135.35美元。在实验2中,将泌乳的多胎泽西母牛提交给Double-Ovsynch协议(DO,n=386),并被随机分配作为未治疗的对照(CON,n=192)或接受2,500IUhCG治疗(hCG,n=194)在ET。ET后7d,hCG的孕酮浓度和总黄体体积大于CON奶牛。与初步实验相反,用hCG治疗在26、33或61d时不影响P/ET,和hCG治疗不影响妊娠损失从26到61天。在接受冷冻/解冻IVP牛肉胚胎的泌乳多胎泽西母牛中,ET后7d接受2,500IUhCG治疗可增加P4浓度和总黄体体积,但不会增加妊娠结局或减少妊娠损失。
    Our objective was to evaluate the effect of treatment with human chorionic gonadotropin (hCG) at the time of transfer of in vitro produced (IVP) beef embryos on pregnancy outcomes in lactating multiparous Jersey cows. Grade 1, Stage 7 (expanded blastocyst), IVP beef embryos were produced from black Angus-based dams using 3 proven high fertility Angus sires and were frozen for direct transfer. In a preliminary experiment, lactating multiparous Jersey cows were randomized to a 2x2 factorial arrangement of treatments to test the main effect of recipient synchronization protocol (Double-Ovsynch; DO; n = 169 vs. a synchronized estrus; ED; n = 180) and were randomly assigned within recipient protocol to serve as untreated controls (DO-CON, n = 78; ED-CON, n = 44) or to receive i.m. treatment with 2,500 IU of hCG (DO-hCG, n = 79; ED-hCG, n = 46) at the time of embryo transfer (ET). The recipient utilization rate was greater for DO (93%) than for ED (50%) cows, and there was an interaction between recipient synchronization protocol and hCG treatment in which DO-hCG cows had more pregnancies per embryo transfer (P/ET) at 26, 33, and 61 d than DO-CON, ED-hCG, and ED-CON cows. Based on a partial budget analysis, the cost per pregnancy for DO cows was $135.35 less than for ED cows. In Experiment 2, lactating multiparous Jersey cows were submitted to a Double-Ovsynch protocol (DO, n = 386) and were randomly assigned to serve as untreated controls (CON, n = 192) or were treated with 2,500 IU hCG (hCG, n = 194) at ET. Progesterone concentrations and total luteal volume 7 d after ET were greater for hCG than for CON cows. In contrast to the preliminary experiment, treatment with hCG did not affect P/ET at 26, 33, or 61 d, and treatment with hCG did not affect pregnancy loss from 26 to 61 d. In conclusion, treatment with 2,500 IU of hCG at ET increased P4 concentrations and total luteal volume 7 d after ET but did not increase pregnancy outcomes or decrease pregnancy loss in lactating multiparous Jersey cows receiving frozen/thawed IVP beef embryos.
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  • 文章类型: Journal Article
    胚胎移植(ET)后单次低剂量重组hCG注射在来曲唑诱导的改良自然冷冻胚胎移植周期(mNC-FET)中的作用是什么?2022年至2024年在大学附属转诊诊所进行了一项观察性研究。包括18-42岁的具有至少一个从先前周期获得的玻璃化胚泡的女性。用口服来曲唑(5mg/天)开始子宫内膜制备的排卵诱导,持续5天。当前导卵泡>17毫米时,使用6500IUrechCGsc触发排卵,子宫内膜厚度>7.5毫米,血清孕酮(P)<1.5ng/ml。所有女性接受30毫克地屈孕酮/天po额外的5天黄体支持。第六天,进行ET。基于准随机设计,一组女性在ET的第3天早晨(hCG组)额外接受了半单次推注(3250IU)rechCG(sc)。没有接受额外hCG的女性被指定为对照。在此期间,在2150个启动的FET周期中,检测到154名妇女符合研究条件。这些群体的人口统计数据,包括平均女性年龄,BMI,血清AMH,和不孕症病因,在变量方面具有可比性。平均血清孕酮值和移植胚胎的数量也相似。hCG组的持续妊娠/开始周期明显高于对照组(分别为46.7%和33.6%,p=0.03*)。ET后单次低剂量hCG注射可能会改善来曲唑mNC-FET周期中女性的OPR。
    What is the effect of a single low-dose recombinant hCG injection after embryo transfer (ET) in letrozole-induced modified natural frozen embryo transfer cycles (mNC-FET)?. An observational study was conducted in the university-affiliated referral clinic between 2022 and 2024. Women aged 18-42 with at least one vitrified blastocyst obtained from the previous cycle(s) were included. Ovulation induction for endometrial preparation was initiated with oral letrozol (5 mg/day) for five days. Ovulation was triggered using 6500 IU rec hCG sc when the leading follicle > 17 mm, endometrial thickness > 7.5 mm, and serum progesterone (P) < 1.5 ng/ml. All women received 30 mg dydrogesterone/day po for additional five-day luteal support. On the 6th day, ET was performed. Based on a quasi-randomized design, a group of women additionally received a half single bolus of (3250 IU) rec hCG (sc) on the morning of 3rd day of ET (hCG group). Women who did not receive additional hCG were assigned as controls. One hundred fifty-four women were detected to be eligible for the study among 2150 initiated FET cycles during the period. Demographic data of the groups, including mean women\'s age, BMI, serum AMH, and infertility etiologies, were comparable in terms of variables. Mean serum progesterone values and the number of transferred embryos were also similar. A significantly higher ongoing pregnancy/started cycle was documented in the hCG group than in controls (46.7% vs 33.6% respectively, p = 0.03*). A single low-dose hCG injection after ET may improve the OPRs of women in letrozole mNC-FET cycles.
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  • 文章类型: Case Reports
    输卵管流产的特征是胎儿挤压到腹腔(腹膜)。它可以是完全挤压或残存在输卵管中的不完全组织。这是一种异位妊娠,很难确定输卵管妊娠的确切发生率。识别输卵管流产病例对于个性化护理至关重要,因为它可以导致更保守的治疗方法。诊断应基于超声成像,在探查手术期间b-hCG水平和视觉构象,开放或腹腔镜。本文描述了一名30岁患者的情况,该患者表现为下腹痛,并因怀疑异位妊娠而入院。超声成像显示子宫旁类似输卵管妊娠的肿块,b-hCG水平为111.8U/L。在腹腔镜手术期间,在道格拉斯(直肠子宫袋)的袋中检测到输卵管流产。这一发现使我们保留了两个输卵管。组织病理学证实了我们的临床表现。在输卵管流产的情况下,保守的方法就足够了,可以保持生育能力和输卵管功能。
    Tubal abortion is characterized by the extrusion of the foetus into the abdominal (peritoneal) cavity. It can either be a complete extrusion or incomplete with residual tissue remaining in the fallopian tube. It is a type of ectopic pregnancy that is difficult to determine the exact incidence of tubal pregnancies. Identifying cases of tubal abortions is crucial for individualized care since it can lead to a more conservative treatment approach. The diagnosis should be based on ultrasound imaging, b-hCG levels and visual conformation during exploratory surgery, either open or laparoscopic. The article describes the case of a 30-year old patient who presented with lower abdominal pain and was admitted for a suspected ectopic pregnancy. Ultrasound imaging showed a mass resembling a tubal pregnancy next to the uterus with b-hCG levels of 111.8 U/L. During laparoscopic surgery, a tubal abortion was detected in the pouch of Douglas (Rectouterine pouch). This finding led us to preserve both fallopian tubes. Histopathology confirmed our clinical findings. A conservative approach can be sufficient in case of tubal abortions, which can lead to preserved fertility and tubal functions.
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  • 文章类型: Journal Article
    目的:人绒毛膜促性腺激素(hCG)在胚胎着床和维持妊娠中起着至关重要的作用。免疫避孕方法包括使用用佐剂indicuspranii分枝杆菌(MIP)配制的重组hCGβ-LTB疫苗,为了防止怀孕而不干扰排卵,荷尔蒙档案,和女性的月经周期。目前在小鼠中的工作旨在解决使用hCGβ-LTB疫苗进行的临床试验中遇到的问题,重点关注两个主要问题。首先,其目的是确定诱导高水平抗hCG抗体所需的最佳疫苗剂量.其次,它旨在评估疫苗的安全性,特别是结节形式的注射部位反应,在一些主题中观察到。
    方法和结果:研究表明,2µg剂量的蛋白质版本的疫苗,通过肌内途径在小鼠中给药,可诱导高抗hCG滴度。此外,施用加强剂量增强抗体应答。我们的发现表明,佐剂MIP的浓度和给药频率也可以降低,而不会损害疫苗的效力。
    结论:注射部位结节形成的问题可以通过肌内注射疫苗和MIP或在不同的皮内部位注射hCG疫苗和MIP来缓解。因此,通过肌内途径以2µg剂量给药的蛋白质疫苗解决了功效和安全性问题.
    在女性中使用重组hCG疫苗启动的I/II期临床试验显示,所有受试者的抗体滴度不足,一些参与者在注射部位出现结节。在小鼠中进行研究以提出减轻注射部位反应和增强抗体反应的潜在策略。结论是获得高抗体滴度的蛋白质版本疫苗的最佳剂量,在坚持安全标准的同时,肌内给药2µg。
    UNASSIGNED: Human Chorionic Gonadotropin (hCG) plays a crucial role in embryo implantation and in maintenance of pregnancy. An immuno-contraceptive approach involves the use of a recombinant hCGβ-LTB vaccine formulated with adjuvant Mycobacterium indicus pranii (MIP), to prevent pregnancy without disturbing ovulation, hormonal profiles, and menstrual cycles in women. The present work in mice was designed to address issues encountered in clinical trials conducted with hCGβ-LTB vaccine, with focus on two primary concerns. Firstly, it aimed to determine the optimal vaccine dosage required to induce a high level of anti-hCG antibodies. Secondly, it aimed to assess the safety profile of the vaccine, specifically injection site reactions in the form of nodules, observed in some of the subjects.
    UNASSIGNED: Studies undertaken indicate that a 2 µg dose of the protein version of the vaccine, administered in mice through the intramuscular route, can induce high anti-hCG titres. Furthermore, administering a booster dose enhances the antibody response. Our findings suggest that the concentration and frequency of administration of the adjuvant MIP can also be reduced without compromising vaccine efficacy.
    UNASSIGNED: The issue of nodule formation at the injection site can be mitigated either by administering the vaccine along with MIP intramuscularly or injecting hCG vaccine and MIP at separate intradermal sites. Thus, protein vaccine administered at a 2µg dose via the intramuscular route addresses both efficacy and safety concerns.
    The Phase I/II clinical trials initiated with the recombinant hCG vaccine in women revealed inadequate antibody titres in all subjects, alongside the development of nodules at the injection sites in some participants. Studies were undertaken in mice to propose potential strategies for mitigating injection site reactions and enhancing the antibody response. It was concluded that the optimum dose of the protein version of the vaccine to get high antibody titres, is 2 µg administered intramuscularly while upholding safety standards.
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  • 文章类型: Journal Article
    背景:普通的马莫西,Callithrixjacchus,是生物医学研究中的宝贵模型。它的用途包括基因工程应用,这需要在体外操作卵母细胞和生产胚胎。为了最大限度地回收适合胚胎生产的卵母细胞,并尽可能最大程度地满足3R原则的要求,优化卵巢刺激方案至关重要。这里,我们比较了两种激素卵巢刺激方法的疗效:1)用hFSH刺激卵泡生长,然后用hCG(FSH+hCG)触发卵母细胞成熟,2)用hFSH刺激(FSH-priming).
    方法:总共,在这项研究中,将14只雌性猕猴用作卵母细胞供体。每只动物都经历了多达四次手术干预,前三个作为卵子拾取(OPU)程序和最后一个是卵巢子宫切除术(OvH)。总的来说,用FSH+hCG刺激进行20个实验,用FSH引发进行18个实验。通过体外成熟(IVM)评估每种刺激方案的功效,体外受精(IVF)和胚胎生产率。
    结果:每个研究组由两个亚组组成:体内成熟卵母细胞和接受IVM的卵母细胞。令人惊讶的是,在没有hCG触发的情况下,一些回收的卵母细胞处于MII期,此外,与FSH+hCG刺激相比,它们的数量并没有显著降低(2.8vs.3.9,分别为(ns))。虽然两个刺激组之间的IVM和IVF率没有差异,在FSH引发组和FSH+hCG组中,体内成熟卵母细胞的IVF率显著低于体外成熟卵母细胞.总的来说,1.7八细胞胚胎/实验(OPU)和2.1八细胞胚胎/实验(OvH)后获得FSH+hCG刺激与1.8FSH引发后的八细胞胚胎/实验(OPU)和5.0八细胞胚胎/实验(OvH)。这些数字包括从体内和体外成熟卵母细胞获得的胚胎。
    结论:体内成熟卵母细胞的发育能力显著降低,使得用hCG作为目前使用的FSH刺激方案的一部分来触发体内成熟。在实际数字中,每次FSH引发后获得1至7个胚泡。在没有进一步研究的情况下,在当前的实验设置下,在普通mar猴中,FSH引发似乎优于FSHhCG刺激。
    BACKGROUND: The common marmoset, Callithrix jacchus, is an invaluable model in biomedical research. Its use includes genetic engineering applications, which require manipulations of oocytes and production of embryos in vitro. To maximize the recovery of oocytes suitable for embryo production and to fulfil the requirements of the 3R principles to the highest degree possible, optimization of ovarian stimulation protocols is crucial. Here, we compared the efficacy of two hormonal ovarian stimulation approaches: 1) stimulation of follicular growth with hFSH followed by triggering of oocyte maturation with hCG (FSH + hCG) and 2) stimulation with hFSH only (FSH-priming).
    METHODS: In total, 14 female marmosets were used as oocyte donors in this study. Each animal underwent up to four surgical interventions, with the first three performed as ovum pick-up (OPU) procedures and the last one being an ovariohysterectomy (OvH). In total, 20 experiments were carried out with FSH + hCG stimulation and 18 with FSH-priming. Efficacy of each stimulation protocol was assessed through in vitro maturation (IVM), in vitro fertilization (IVF) and embryo production rates.
    RESULTS: Each study group consisted of two subgroups: the in vivo matured oocytes and the oocytes that underwent IVM. Surprisingly, in the absence of hCG triggering some of the oocytes recovered were at the MII stage, moreover, their number was not significantly lower compared to FSH + hCG stimulation (2.8 vs. 3.9, respectively (ns)). While the IVM and IVF rates did not differ between the two stimulation groups, the IVF rates of in vivo matured oocytes were significantly lower compared to in vitro matured ones in both FSH-priming and FSH + hCG groups. In total, 1.7 eight-cell embryos/experiment (OPU) and 2.1 eight-cell embryos/experiment (OvH) were obtained after FSH + hCG stimulation vs. 1.8 eight-cell embryos/experiment (OPU) and 5.0 eight-cell embryos/experiment (OvH) following FSH-priming. These numbers include embryos obtained from both in vivo and in vitro matured oocytes.
    CONCLUSIONS: A significantly lower developmental competence of the in vivo matured oocytes renders triggering of the in vivo maturation with hCG as a part of the currently used FSH-stimulation protocol unnecessary. In actual numbers, between 1 and 7 blastocysts were obtained following each FSH-priming. In the absence of further studies, FSH-priming appears superior to FSH + hCG stimulation in the common marmoset under current experimental settings.
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  • 文章类型: Journal Article
    比较使用不同rFSH选择的体外受精(IVF)/卵胞浆内单精子注射(ICSI)(IVF/ICSI)治疗的老年人群的卵母细胞数量和排卵诱导的临床结果以及使用促性腺激素释放激素(GnRH-a)与人绒毛膜促性腺激素(HCG)引发剂的拮抗剂治疗诱导排卵的有效性。
    本研究共选择了132个新鲜周期,2022年3月至2022年12月在我们医院接受IVF/ICSI治疗。根据不同的亚组和不同的触发方法对获得的卵母细胞数量的影响进行观察,胚胎质量,和临床结果。
    促性腺激素(Gn)的初始剂量,卵母细胞的数量,A组MII卵母细胞数高于B组(p<0.05),A组临床妊娠率为29.41%,B组临床妊娠率为27.5%。在2PN的数量方面,双触发组优于HCG触发组,存活胚胎的数量,和高质量胚胎的数量(p<0.05)。使用双触发方案(OR=0.667,95CI(0.375,1.706),p=.024)是临床妊娠率的保护因素,而AFC(OR=0.925,95CI(0.867,0.986),p=.017)是临床妊娠率的独立因素。
    GnRH-a与HCG联合使用适当的拮抗剂可改善老年患者新鲜胚胎移植周期的妊娠结局。
    UNASSIGNED: To compare the number of oocytes retrieved and clinical outcomes of ovulation induction in an older population treated with in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) (IVF/ICSI) using different rFSH options and the effectiveness of antagonist treatment to induce ovulation using gonadotropin-releasing hormone agonists (GnRH-a) in combination with an human chorionic gonadotropin (HCG) trigger.
    UNASSIGNED: A total of 132 fresh cycles were selected for this study, which were treated with IVF/ICSI in our hospital from March 2022 to December 2022. Observations were made according to different subgroups and the effects of different triggering methods on the number of oocytes obtained, embryo quality, and clinical outcomes.
    UNASSIGNED: The initial gonadotropin (Gn) dose, the number of oocytes, and the number of MII oocytes were higher in group A than in group B (p < .05), and the clinical pregnancy rate was 29.41% in group A. Group B had a clinical pregnancy rate of 27.5%. The double-trigger group was superior to the HCG-trigger group in terms of the number of 2PN, the number of viable embryos, and the number of high-quality embryos (p < .05). The use of a double-trigger regimen (OR = 0.667, 95%CI (0.375, 1.706), p = .024) was a protective factor for the clinical pregnancy rate, whereas AFC (OR = 0.925, 95%CI (0.867, 0.986), p = .017) was an independent factor for the clinical pregnancy rate.
    UNASSIGNED: The use of a dual-trigger regimen of GnRH-a in combination with HCG using an appropriate antagonist improves pregnancy outcomes in fresh embryo transfer cycles in older patients.
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  • 文章类型: Journal Article
    黄体(CL)是合成孕酮的临时内分泌腺。黄体孕酮在调节发情周期以及植入和维持妊娠中起着核心作用。我们之前的研究显示了adropin及其受体的表达,GPR19,在黄体细胞中及其在黄体化中的显著感化。本研究的目的是研究adropin对成年小鼠hCG诱导的卵巢功能的体外影响。我们还评估了adropin外源性治疗对卵巢类固醇生成和抗氧化参数的影响,特别强调CL功能。我们的结果表明,adropin与hCG协同作用,通过增加StAR的表达来促进卵巢类固醇生成和存活。3β-HSD,和芳香化酶蛋白并降低BAX/BCL2比率。外源性adropin处理通过增加小鼠卵巢中StAR和3β-HSD酶的表达来增加孕酮的产生。此外,adropin,通过GPR19,通过增加CL中NRF-2的核易位来抑制黄体氧化应激,导致HO-1表达和SOD增加,过氧化氢酶活性。氧化应激降低可能抑制NF-κB向黄细胞核的转位,导致存活率增加和细胞凋亡减少,脂质过氧化作用减少,BAX/BCL2比值,caspase3,活性caspase3表达,和adropin处理的小鼠中的TUNEL阳性细胞。我们的研究结果表明,adropin可能是一种有希望的候选药物,可以延长CL的寿命,并可能作为治疗黄体功能不全的潜在治疗选择。
    The corpus luteum (CL) is a temporary endocrine gland that synthesizes progesterone. The luteal progesterone plays a central role in the regulation of the estrous cycle as well as the implantation and maintenance of pregnancy. Our previous study showed the expression of adropin and its receptor, GPR19, in the luteal cells and its significant role in luteinization. The aim of the present study was to investigate the in vitro effect of adropin on hCG-induced ovarian functions in adult mice. We also evaluated the effect of exogenous treatment with adropin on ovarian steroidogenesis and anti-oxidant parameters, with special emphasis on CL function. Our results demonstrated that adropin acts synergistically with hCG to promote ovarian steroidogenesis and survival by increasing the expression of StAR, 3β-HSD, and aromatase proteins and decreasing the BAX/BCL2 ratio. Exogenous adropin treatment increased progesterone production by increasing the expression of GPR19, StAR and 3β-HSD enzymes in the mouse ovary. Also, adropin inhibited the luteal oxidative stress by increasing nuclear translocation of NRF-2 in CL, which resulted in increased HO-1 expression and SOD, catalase activity. Decreased oxidative stress might inhibit the translocation of NF-κB into the nucleus of luteal cells, resulting into increased survival and decreased apoptosis, as evident by decreased lipid peroxidation, BAX/BCL2 ratio, caspase 3, active caspase 3 expression, and TUNEL-positive cells in adropin treated mice. Our findings suggest that adropin can be a promising candidate that can enhance the survivability of the CL.
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  • 文章类型: Journal Article
    该研究检验了以下假设:单次施用hCG支持在乳晕期间同步绵羊的终末卵泡发育的LH依赖性阶段,使用eCG作为功能参考。使用临床方法,设计了四个实验来实现以下目标:(1)确定发情对生殖效率的抑制作用;(2)评估单剂量后hCG保持功能血液浓度的潜力;(3)表征不同剂量的hCG对生殖功能标志物的影响;(4)根据相同的标志物比较hCG与eCG支持卵泡发育和生育力的能力。结果表明,由于FSH依赖性标志物未受到损害,因此在LH依赖性下,急流似乎会影响卵泡和黄体功能;hCG保持比对照组更高的血液浓度至少48小时;与对照组相比,在与繁殖季节相当的标准下,hCG改善了卵泡发育和排卵率;用hCG处理的母羊表现与用eCG处理的母羊相似。我们的结果得出结论,hCG可用于支持绵羊在发情期的卵泡功能,旨在完善其在辅助生殖中的调控。
    The study tested the hypothesis that a single administration of hCG supports the LH-dependent phase of terminal follicular development in synchronized sheep during anestrus, using eCG as a functional reference. Using a clinical approach, four experiments were designed to achieve the following: (1) Identify the inhibitory influence of anestrus on reproduction efficiency; (2) Assess the potential of hCG to keep functional blood concentrations after a single dose; (3) Characterize the effect of different doses of hCG on reproductive functional markers; (4) To compare the ability of hCG to that of eCG to support follicular development and fertility based on the same markers. The results showed that anestrus seems to affect follicular and luteal function under LH dependency as FSH-dependent markers are not compromised; hCG maintains higher blood concentrations than controls for at least 48 h; hCG improves follicular development and ovulatory rates compared to controls and at standards comparable to a breeding season; and ewes treated with hCG exhibit similar performance to those treated with eCG. Our results conclude that hCG can be used to support follicular function during anestrus in sheep, aiming to perfect its regulation in assisted reproduction.
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  • 文章类型: Journal Article
    我们假设hCG在妊娠早期调节牛子宫内膜中IFNT途径和ISG的表达。本研究的目的是评估hCG对子宫内膜细胞中IFNT途径信号和ISGs表达的影响。为此,在研究中使用了29头非泌乳交叉面包母牛,并接受了9天固定时间的人工授精(FTAI)方案。AI的日期被认为是第0天(D0),FTAI后五天(D5),将奶牛分为两组:对照组和hCG组,当hCG组接受单剂量2.500UI的hCG时。在FTAI(D18)后第18天,屠宰奶牛并收集子宫内膜组织样品。C中奶牛的胚胎恢复率与hCG相比没有差异。hCG组增加了副黄体形成率。与第14天的C相比,hCG在hCG组中导致更高的血清孕酮浓度。与C组相比,仅IFNAR2和STAT1的表达在hCG组的妊娠母牛上上调。通路基因(JAK1、STAT2和IRF9)不受调控。hCG组妊娠奶牛中ISG15、MX1、MX2和OAS1的mRNA丰度上调,与C组相比。结果表明,HCG的给药,AI后5天,除了增加血清孕酮,在妊娠第18天调节牛子宫内膜上IFNT途径和ISGs的表达。
    We hypothesized that the hCG modulates the expression of IFNT-pathway and ISGs in bovine endometrium during early pregnancy. The aim of the current study is to evaluate the effect of hCG on IFNT-pathway signals and ISGs expression in endometrial cells. For this, 29 non-lactating cross-bread cows were used in the study and submitted to a 9-day fixed-time artificial insemination (FTAI) protocol. The day of the AI was considered Day 0 (D0), and five days (D5) after the FTAI, the cows were allocated into two groups: Control and hCG group, when a hCG group received a single dose of 2.500UI of hCG. On day 18 after FTAI (D18) cows were slaughtered and endometrial tissue samples were collected. There was no difference between the embryo recovery rate of the cows in C compared to the hCG. The hCG group increased the accessory corpus luteum formation rate. The hCG resulted in greater serum progesterone concentration in the hCG group compared to the C on Day 14. Only the expression of IFNAR2 and STAT1 were upregulated on pregnant cows of the hCG group compared to the C group. The pathway genes (JAK1, STAT2, and IRF9) were not regulated. The mRNA abundance of ISG15, MX1, MX2, and OAS1 was upregulated in pregnant cows for hCG group, compared to C group. The results show that the administration of hCG, 5 days after AI, in addition to increasing the serum progesterone, modulates the expression of IFNT-pathway and ISGs on bovine endometrium on Day 18 of pregnancy.
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