PDF(Pubmed)
Abstract：
The study tested the hypothesis that a single administration of hCG supports the LH-dependent phase of terminal follicular development in synchronized sheep during anestrus, using eCG as a functional reference. Using a clinical approach, four experiments were designed to achieve the following: (1) Identify the inhibitory influence of anestrus on reproduction efficiency; (2) Assess the potential of hCG to keep functional blood concentrations after a single dose; (3) Characterize the effect of different doses of hCG on reproductive functional markers; (4) To compare the ability of hCG to that of eCG to support follicular development and fertility based on the same markers. The results showed that anestrus seems to affect follicular and luteal function under LH dependency as FSH-dependent markers are not compromised; hCG maintains higher blood concentrations than controls for at least 48 h; hCG improves follicular development and ovulatory rates compared to controls and at standards comparable to a breeding season; and ewes treated with hCG exhibit similar performance to those treated with eCG. Our results conclude that hCG can be used to support follicular function during anestrus in sheep, aiming to perfect its regulation in assisted reproduction.
摘要：
该研究检验了以下假设：单次施用hCG支持在乳晕期间同步绵羊的终末卵泡发育的LH依赖性阶段，使用eCG作为功能参考。使用临床方法，设计了四个实验来实现以下目标：（1）确定发情对生殖效率的抑制作用；（2）评估单剂量后hCG保持功能血液浓度的潜力；（3）表征不同剂量的hCG对生殖功能标志物的影响；（4）根据相同的标志物比较hCG与eCG支持卵泡发育和生育力的能力。结果表明，由于FSH依赖性标志物未受到损害，因此在LH依赖性下，急流似乎会影响卵泡和黄体功能；hCG保持比对照组更高的血液浓度至少48小时；与对照组相比，在与繁殖季节相当的标准下，hCG改善了卵泡发育和排卵率；用hCG处理的母羊表现与用eCG处理的母羊相似。我们的结果得出结论，hCG可用于支持绵羊在发情期的卵泡功能，旨在完善其在辅助生殖中的调控。
