在Riaño和Mampodre的区域狩猎保护区中进行了of的胃肠道线虫研究,莱昂省,西班牙,提供与采样区域相关的感染率和感染强度的信息,动物的年龄,和体重。通过对动物进行规范的尸检,它们的消化道都有胃肠线虫,平均寄生强度为638±646.1线虫/感染动物。发现胃肠线虫11属,鉴定出18种,其中三个是多态的:赤线菌,玻璃化毛线体,无核毛线菌,胶毛刚毛,Haemonchuscontrortus,鳞翅目/鳞翅目,Ostertagialeptospicularis/Ostertagiakolchida,奥斯特塔吉(Grosspiculopteragia)occidentalis,Teladorsagiacircincta/Teladorsagiatrifurcate,MarshallagiaMarshalli,欧洲线虫,Cooperia蛇,牛毛细血管,静脉食管造口,还有毛鞭毛.所有这些都已经在欧洲的ro中被引用过,但是MarshallagiaMarshalli,牛毛细血管,西班牙首次报道了Ostertagia(Grosspiculopteragia)occidentalis。皱胃是肠道,其中患病率(98.9%)和平均强度(x'=370.7±374.4蠕虫/ro;范围3-1762)显着更高,但是在比较动物的采样面积和年龄时,没有发现统计学上的显着差异。体重较低的动物比身体状况良好的动物有更高的寄生虫负荷,finding,在这种情况下,统计学差异显著(p=0.0020)。鉴定出7属和14种。在小肠里,88%的受检动物表现为胃肠线虫,平均强度为x=131.7±225.6寄生虫/受感染的动物,范围在4-1254个蠕虫之间。比较研究的三个参数时,没有发现统计学上的显着差异。确定了四个属和七个物种。在大肠/盲肠中,78.3%的受检鹿出现成虫,平均强度为6.3±5.5个蠕虫/感染动物;范围为1-26个蠕虫。考虑到寄生的平均强度和采样面积(p=0.0093),仅观察到统计学上的显着差异。鉴定了两个属和两个物种。对研究中发现的几种物种进行了分子研究,并将获得的序列与存放在GenBank中的序列进行比较,准备了系统发育树以确定其分类状态。使用协同逻辑技术,在同一研究区域中,研究了ro中胃肠道线虫卵脱落与半广泛绵羊养殖场的相关性,以验证这些寄生虫在野生和家畜之间的交叉传播。在研究参数中发现的高值表明,西班牙北部是the的高强度感染地区。
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study of gastrointestinal nematodes in roe deer was carried out in the regional hunting reserves of Riaño and Mampodre, Province of León, Spain, to provide information on their prevalence and intensity of infection in relation to the sampling areas, age of the animals, and body weight. Through a regulated necropsy of the animals, all of them harbored gastrointestinal nematodes in their digestive tract, with a mean intensity of parasitism of 638 ± 646.1 nematodes/infected animal. Eleven genera were found and 18 species of gastrointestinal nematodes were identified, three of them polymorphic: Trichostrongylus axei, Trichostrongylus vitrinus, Trichostrongylus capricola, Trichostrongylus colubriformis, Haemonchus contortus, Spiculopteragia spiculoptera/Spiculopteragia mathevossiani, Ostertagia leptospicularis/Ostertagia kolchida, Ostertagia (Grosspiculopteragia) occidentalis, Teladorsagia circumcincta/Teladorsagia trifurcate, Marshallagia marshalli, Nematodirus europaeus, Cooperia oncophora, Capillaria bovis, Oesophagostomum venulosum, and Trichuris ovis. All of them have already been cited in roe deer in Europe, but Marshallagia marshalli, Capillaria bovis, and Ostertagia (Grosspiculopteragia) occidentalis are reported for the first time in Spain in this host. The abomasum was the intestinal section, where the prevalence (98.9%) and mean intensity (x¯ = 370.7 ± 374.4 worms/roe deer; range 3-1762) were significantly higher, but no statistically significant differences were found when comparing the sampling areas and age of animals. The animals with lower body weight had a higher parasite load than those in better physical condition, finding, in this case, statistically significant differences (p = 0.0020). Seven genera and 14 species were identified. In the small intestine, 88% of the animals examined presented gastrointestinal nematodes, with an average intensity of x¯ = 131.7 ± 225.6 parasites/infected animal, ranging between 4-1254 worms. No statistically significant differences were found when the three parameters studied were compared. Four genera and seven species were identified. In the large intestine/cecum, 78.3% of the examined roe deer presented adult worms, with an average intensity of 6.3 ± 5.5 worms/infected animal; range 1-26 worms. Only statistically significant differences were observed when considering the mean intensity of parasitism and the sampling area (p = 0.0093). Two genera and two species were identified. Several of the species found in the
study were studied molecularly, and with the sequences obtained compared with those deposited in GenBank, phylogenetic trees were prepared to determine their taxonomic status. Using coprological techniques, the existing correlation in the shedding of gastrointestinal nematode eggs in roe deer was investigated with that of semi-extensive sheep farms in the same
study area to verify the existence of cross-transmission of these parasites between wild and domestic animals. The high values found in the studied parameters show that northern Spain is an area of high-intensity infection for roe deer.