基于聚酯的支架对于各种组织的再生具有研究意义。然而,需要改善支架润湿性并引入生物活性。表面改性是一种广泛研究的方法,用于改善支架性能并保持适当的整体性能。在这项研究中,比较了使用明胶固定化使聚(丙交酯-共-ε-己内酯)PLCL纤维的表面官能化的三种方法。水解,氧等离子体处理,选择氨解作为活化方法,在明胶固定化之前在表面上引入羧基(-COOH)和氨基(-NH2)官能团。共价连接明胶,选择碳二亚胺偶联用于水解和等离子体处理的材料,在氨基分解样品的情况下使用戊二醛交联。制备明胶的物理包埋和使用碳二亚胺偶联固定而没有预先活化的材料作为对照。表面上明胶量的差异,对纤维形态的影响,分子量,根据改性的类型和活化的应用参数,观察到机械性能。结果表明,水解对材料表面的影响最大,而等离子体处理和氨解对材料的整个体积有影响。尽管有这种差异,所有方法的整体机械性能都受到影响。所有材料在官能化之后是完全亲水的。对于任何样品均未发现细胞毒性。明胶固定化导致改善的L929细胞形态,对于用水解和等离子体处理活化的样品具有最佳效果。我们的研究表明,任何表面活化方法的使用都应限于最低的浓度/反应时间,以实现随后令人满意的功能化,并且该决定应基于最终支架材料必须执行的特定功能。
Polyester-based scaffolds are of research interest for the regeneration of a wide spectrum of tissues. However, there is a need to improve scaffold wettability and introduce bioactivity. Surface modification is a widely studied approach for improving scaffold performance and maintaining appropriate bulk properties. In this
study, three methods to functionalize the surface of the poly(lactide-co-ε-caprolactone) PLCL fibres using gelatin immobilisation were compared. Hydrolysis, oxygen plasma treatment, and aminolysis were chosen as activation methods to introduce carboxyl (-COOH) and amino (-NH2) functional groups on the surface before gelatin immobilisation. To covalently attach the gelatin, carbodiimide coupling was chosen for hydrolysed and plasma-treated materials, and glutaraldehyde crosslinking was used in the case of the aminolysed samples. Materials after physical entrapment of gelatin and immobilisation using carbodiimide coupling without previous activation were prepared as controls. The difference in gelatin amount on the surface, impact on the fibres morphology, molecular weight, and mechanical properties were observed depending on the type of modification and applied parameters of activation. It was shown that hydrolysis influences the surface of the material the most, whereas plasma treatment and aminolysis have an effect on the whole volume of the material. Despite this difference, bulk mechanical properties were affected for all the approaches. All materials were completely hydrophilic after
functionalization. Cytotoxicity was not recognized for any of the samples. Gelatin immobilisation resulted in improved L929 cell morphology with the best effect for samples activated with hydrolysis and plasma treatment. Our
study indicates that the use of any surface activation method should be limited to the lowest concentration/reaction time that enables subsequent satisfactory
functionalization and the decision should be based on a specific function that the final scaffold material has to perform.