背景:H.syriacus\“dansiban\”的花色从紫红色过渡到粉红色紫色,最后过渡到浅紫色,从而提高品种的观赏价值。然而,H.syriacus花色变化的分子机制尚未阐明。在这项研究中,分析了H.syriacus\'dansiban\'在五个发育阶段的转录组数据,以研究类黄酮成分对花颜色变化的影响。此外,在关键的开花阶段,从H.syriacus\'dansiban\'构建了五个cDNA文库,并对转录组进行测序,以研究花朵颜色变化的分子机制。
结果:高效液相色谱-质谱法检测到了H.syriacus\'千思班\'中的五种花色苷,malvaccin-3-O-葡萄糖苷是不同阶段H.syriacus花中的主要化合物,其次是petunigenin-3-O-葡萄糖苷。在整个开花过程中,这五种花色苷的水平逐渐下降。在类黄酮和黄酮醇的组成和概况方面,总共鉴定出7种类黄酮:槲皮素-3-葡萄糖苷,木犀草素-7-O-葡萄糖苷,santianol-7-O-葡萄糖苷,山奈酚-O-己糖-C-己糖苷,芹菜素-C-二葡萄糖苷,木犀草素-3,7-二葡萄糖苷,和芹菜素-7-O-rutinoside.基于所选择的参考基因组鉴定了总共2,702个DEG。基于差异表达基因的富集分析,我们确定了9个结构基因(PAL,CHS,FLS,DRF,ANS,CHI,F3H,F3\'5\'H,和UFGT)和7个转录因子(3MYB,4bHLH)与类黄酮生物合成相关。qRT-PCR结果与高通量测序数据吻合良好。
结论:这项研究将为阐明H.syriacus花色素沉着改变的潜在机制奠定基础。
BACKGROUND: The flower colour of H. syriacus \'Qiansiban\' transitions from fuchsia to pink-purple and finally to pale purple, thereby enhancing the ornamental value of the cultivars. However, the molecular mechanism underlying this change in flower colour in H. syriacus has not been elucidated. In this
study, the transcriptomic data of H. syriacus \'Qiansiban\' at five developmental stages were analysed to investigate the impact of
flavonoid components on flower colour variation. Additionally, five cDNA libraries were constructed from H. syriacus \'Qiansiban\' during critical blooming stages, and the transcriptomes were sequenced to investigate the molecular mechanisms underlying changes in flower colouration.
RESULTS: High-performance liquid chromatography‒mass spectrometry detected five anthocyanins in H. syriacus \'Qiansiban\', with malvaccin-3-O-glucoside being the predominant compound in the flowers of H. syriacus at different stages, followed by petunigenin-3-O-glucoside. The levels of these five anthocyanins exhibited gradual declines throughout the flowering process. In terms of the composition and profile of flavonoids and flavonols, a total of seven flavonoids were identified: quercetin-3-glucoside, luteolin-7-O-glucoside, Santianol-7-O-glucoside, kaempferol-O-hexosyl-C-hexarbonoside, apigenin-C-diglucoside, luteolin-3,7-diglucoside, and apigenin-7-O-rutinoside. A total of 2,702 DEGs were identified based on the selected reference genome. Based on the enrichment analysis of differentially expressed genes, we identified 9 structural genes (PAL, CHS, FLS, DRF, ANS, CHI, F3H, F3\'5\'H, and UFGT) and 7 transcription factors (3 MYB, 4 bHLH) associated with
flavonoid biosynthesis. The qRT‒PCR results were in good agreement with the high-throughput sequencing data.
CONCLUSIONS: This
study will establish a fundamental basis for elucidating the mechanisms underlying alterations in the flower pigmentation of H. syriacus.