背景:具有丰富的抗坏血酸含量的植物提取物具有更大的抗氧化能力;广泛用于皮肤美容产品并保护皮肤免受有害的光损伤环境效应。甘蓝科具有很强的抗氧化活性,因此对药妆具有广阔的前景。
目的:研制一种具有显著抗氧化活性的甘蓝叶提取物外用乙醇凝胶。
方法:2,2-二苯基-1-吡啶酰肼(DPPH)对植物叶提取物抗氧化能力的评价,通过高效液相色谱(HPLC)对抗坏血酸进行定量。采用冷法制备醇质体。将含有提取物的优化悬浮液与提取物溶液(对照)一起掺入2%Carbopol凝胶(测试)中。对最终产品进行非侵入性体内研究,以通过测量黑色素和红斑来评估其对皮肤的影响。皮脂水平,弹性,湿度等级,面部毛孔的数量和面积,皮肤起皱,和平滑度。
结果:甘蓝叶提取物在14.22μg/g抗坏血酸中表现出显着的抗氧化潜力(85.64±1.28%);在皮肤黑色素方面表现出突出的美容效果,红斑,皮脂,弹性,水合作用,面部毛孔,皱纹,和平滑时,掺入乙醇体。ANOVA检验对皮肤也表现出显著的正效应(p≤0.05)。
结论:甘蓝提取物是一种强抗氧化剂,对皮肤具有显著的皮肤美容功效。
BACKGROUND: Plant extracts with rich ascorbic acid contents have greater antioxidant capability; extensively employed in skin beautifying products and protect skin from detrimental photodamaging environmental effects. Brassica oleraceae is having a substantial prospective toward cosmeceuticals owed by its profound activity against oxidation.
OBJECTIVE: To develop an effective topical ethosomal gel loaded with Brassica oleraceae leaves extract with significant antioxidant activity.
METHODS: Valuation of antioxidant capability of plant leaves extract by 2,2-diphenyl-1-picrylhydrazyl (DPPH), and quantification of ascorbic acid was done through high performance liquid chromatography (HPLC).
Ethosomes were prepared by cold method. Optimized suspension containing extract was incorporated in 2% Carbopol gel (test) along with extract solution (control). Noninvasive in vivo studies were performed for final product to assess its effects on skin by measuring melanin and erythema, sebum level, elasticity, moistness level, facial pores count and their area, skin wrinkling, and smoothness.
RESULTS: Brassica oleraceae (red cabbage) leaves extract exhibited significant antioxidant potential (85.64 ± 1.28%) with 14.22 μg/g of ascorbic acid; expressed prominent cosmetic effects in terms of skin melanin, erythema, sebum, elasticity, hydration, facial pores, wrinkles, and smoothness when incorporated in
ethosomes. ANOVA test also exhibited positive significant (p ≤ 0.05) effects on skin.
CONCLUSIONS: Brassica oleraceae extract is a strong antioxidant with remarkable dermocosmetic benefits for skin.