通过应激颗粒的组装来控制整体蛋白质合成代表了真核细胞面对各种应激条件所采用的策略。TIA1相关核溶素(TIAR),曲司曲普林(TTP),Ras-GTP酶激活蛋白SH3结构域结合蛋白(G3BP)是应激颗粒的关键成分,允许调节mRNA的稳定性,从而不仅控制应激反应,而且控制细胞增殖和分化。在这项研究中,我们的目的是调查蒂尔的角色,ttp,在生理和胁迫条件下,孤立海鞘Cionarobusta的胚胎发育过程中和g3bp。我们进行了CRISPR/Cas9来评估基因敲除对正常胚胎发育的影响。和基因报告基因测定来研究基因转录的时间和组织特异性,以及全装原位杂交和定量实时PCR。诱发急性应激状态,我们使用铁和镉作为“必需”和“非必需”金属,分别。我们的结果强调,第一次,提尔的重要性,ttp,和g3bp在无脊椎动物脊索动物胚胎发育过程中控制中内胚层组织衍生物的发育。
Controlling global protein synthesis through the assembly of stress granules represents a strategy adopted by eukaryotic cells to face various stress conditions. TIA 1-related nucleolysin (TIAR), tristetraprolin (TTP), and Ras-GTPase-activating protein SH3-domain-binding protein (G3BP) are key components of stress granules, allowing the regulation of mRNA stability, and thus controlling not only stress responses but also cell proliferation and differentiation. In this study, we aimed at investigating the roles of tiar, ttp, and g3bp during
embryogenesis of the solitary ascidian Ciona robusta under both physiological and stress conditions. We carried out CRISPR/Cas9 to evaluate the effects of gene knockout on normal embryonic development, and gene reporter assay to study the time and tissue specificity of gene transcription, together with whole-mount in situ hybridization and quantitative real time PCR. To induce acute stress conditions, we used iron and cadmium as \"essential\" and \"non-essential\" metals, respectively. Our results highlight, for the first time, the importance of tiar, ttp, and g3bp in controlling the development of mesendodermal tissue derivatives during
embryogenesis of an invertebrate chordate.